Cytokine measurement using cytometric bead arrays

Methods in Molecular Biology
Luis Castillo, Donna M MacCallum

Abstract

Cytokines can be measured by enzyme-linked immunosorbent assay (ELISA) or multiplex assay. Both techniques are commonly used in immunology to detect the presence of antibody or antigen in a sample. However, multiplex bead array technology provides the means to simultaneously measure multiple analytes in a single reaction, thereby saving time and resources. This method can detect up to 30 proteins at once, using a relatively small sample volume, without losing sensitivity, accuracy, or reproducibility. In this chapter, we describe the cytometric bead array (CBA) approach to simultaneously measure multiple cytokines in biological samples such as spleen, kidney, or serum from mice infected with the human fungal pathogen Candida albicans.

Citations

Dec 18, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Geneviève Dubeau-LaraméeLuchino Y Cohen
Oct 24, 2013·Cytometry. Part B, Clinical Cytometry·A GrelierH Merle-Beral
Sep 13, 2019·Journal of Immunology Research·Rudolf KäferAndrea Pautz
Apr 21, 2020·International Immunopharmacology·Mengmeng YouFuliang Hu
Mar 27, 2021·Indian Journal of Dermatology, Venereology and Leprology·Fátima Regina Vilani-MorenoMarcos da Cunha Lopes Virmond

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