Cytokine mRNA expression in an in vitro human skin model, SKIN(2)

Toxicology in Vitro : an International Journal Published in Association with BIBRA
F G BurlesonG F Gerberick

Abstract

SKIN(2) ZK1300 is a three-dimensional human skin model consisting of multilayered dermal fibroblasts and well-differentiated epidermal keratinocyte layers, including a stratum corneum. To characterize this model better, constitutive levels of cytokine gene expression were determined. Reverse transcriptase-polymerase chain reaction (RT-PCR), followed by liquid hybridization to labelled internal probes, demonstrated that interleukin (IL)-1I, IL-1beta, IL-6, IL-8, IL-10, tumour necrosis factor (TNF)I, granulocyte macrophage-colony stimulating factor (GM-CSF), transforming growth factor (TGFbeta1) and IL-12 p35 mRNAs were constitutively expressed whereas IL-12 p40 was not. The contribution of the dermal component of this human skin model (Model ZK1100) was further characterized by determining constitutive cytokines expressed and their modulation by phorbol 12-myristate, 13-acetate (PMA). The dermal component, consisting of multilayered human dermal fibroblasts, constitutively expressed message for IL-1I, 1L-1beta, IL-6, IL-8, TGFbeta1, GM-CSF and IL-12 p35. Message was not detected for IL-10, TNFI or IL-12 p40. PMA treatment of the multilayered dermal fibroblasts increased steady-state mRNA levels of IL-1I, IL-1beta, IL-6, IL-8, GM...Continue Reading

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Citations

Apr 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L P BernhoferK M Martin
Apr 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L P BernhoferK M Martin
Dec 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A CoquetteY Poumay
Jun 11, 1998·American Journal of Contact Dermatitis : Official Journal of the American Contact Dermatitis Society·G F Gerberick, E E Sikorski
Mar 1, 1999·Alternatives to Laboratory Animals : ATLA·E CorsiniC L Galli

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