Cytokine mRNA expression in chronically rejected human renal allografts

Clinical Transplantation
Arcangelo NoceraUmberto Valente

Abstract

Although both immunologic and non-immunologic components may cause kidney allograft chronic rejection (KGCR), also referred to as chronic allograft nephropathy (CAN), its pathogenesis is largely not yet understood. To explore relevant immunologic mechanisms occurring in KGCR, we have analyzed in surgically removed KG the transcription of the following cytokine and apoptotic molecule genes: interleukin (IL)-2, IL-3, IL-4, IL-5, IL-6, IL-10, tumor necrosis factor (TNF)-alpha, IFN-gamma, FAS, and FAS-L. Semiquantitative RT-PCR was used and KG explants were obtained from two groups of transplanted patients. Group 1 was represented by CR/CAN KG, removed for: (a) superimposed symptoms of acute lesions (SAL) due to tapering or suspension of immunosuppression (subgroup 1a, eight cases); (b) causes other than SAL (two cases, subgroup 1b). Group 2 comprised explanted kidneys with no CR/CAN (three cases--vascular thrombosis, intrarenal hemorrhage and vascular thrombosis). The results showed that in group 1 IL- 6 was detectable in seven of 10, IL-10 in six of 10, IFN-gamma in five of 10, and IL-3 in four of 10 cases with a variable pattern of reciprocal association. IL-2 and TNF-alpha were represented in one of 10 cases only. Particularly,...Continue Reading

References

Jul 1, 1994·Kidney International·T H Hostetter
Apr 1, 1993·Transplantation Proceedings·L C Paul
Jun 25, 1998·Transplant International : Official Journal of the European Society for Organ Transplantation·C C Baan, W Weimar
Dec 20, 1999·Japanese Circulation Journal·Y FurukawaS Sasayama
Apr 25, 2003·Transplant International : Official Journal of the European Society for Organ Transplantation·Anna MhoyanHaval Shirwan

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Citations

May 1, 2010·Journal of Periodontology·Murad ShaqmanAnna Dongari-Bagtzoglou
Dec 2, 2006·Transplant Immunology·P J Yates, M L Nicholson
Feb 15, 2013·Immunological Reviews·Zhongmin LiuShuiping Jiang
Sep 27, 2005·Transplantation Proceedings·A NoceraU Valente
May 28, 2019·Cytokine & Growth Factor Reviews·Nicolas SaillietSophie Brouard

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