Cytokine mRNA expression in hepatitis C virus infection: TH1 predominance in patients with chronic hepatitis C and TH1-TH2 cytokine profile in subjects with self-limited disease

Journal of Viral Hepatitis
E GigiV Tsapas

Abstract

Many determinants of the immune response have been implied in the pathogenesis of chronic hepatitis C. TH1 and TH2 cytokines play a prominent role in viral infections and a dysregulation of these cytokines could account for viral persistence and evolution of chronic disease. To explore a possible TH1 and TH2 cytokine dysregulation resulting in the inability to terminate hepatitis C virus (HCV) infection, we studied TH1 [interferon (IFN)-gamma, interleukin (IL)-2] and TH2 (IL-4, IL-10) mRNA expression of peripheral blood mononuclear cells (PBMC) in response to NS3 HCV antigen stimulation, in 31 untreated patients with chronic hepatitis C and 29 subjects with self-limited disease. After a 48 h culture of PBMC, total RNA isolation was performed and complementary DNA was prepared by reverse transcription. mRNA levels were quantified by real-time polymerase chain reaction using a standard curve formed after cloning each cytokine gene and a reference gene using recombinant DNA technology in a specific plasmid vector. In the patients group, mRNA expression of IFN-gamma, IL-2 and IL-4 but not IL-10 was detected, IFN-gamma being the predominant cytokine expressed. All four cytokines were expressed in subjects with self limited disease, ...Continue Reading

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Citations

Jun 25, 2013·Cytokine & Growth Factor Reviews·Hong-Liang ZhangJie Zhu
Jan 20, 2016·Disease Markers·Hoda Mohamed El-EmshatyIbrahim Eldsoky Mohamed
Jul 15, 2016·Journal of Immunology Research·Ehab H MattarElrashdy M Redwan
Oct 3, 2012·Clinics and Research in Hepatology and Gastroenterology·Petros C BeniasNeil D Theise
Aug 30, 2016·World Journal of Gastroenterology : WJG·Mahmoud Fathy DondetiRoba Mohamed Talaat
Sep 5, 2019·Frontiers in Neuroscience·Chunrong LiKangding Liu

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