Cytokine mRNA expression in Mycobacterium ulcerans-infected human skin and correlation with local inflammatory response.

Infection and Immunity
Richard PhillipsMark Wansbrough-Jones

Abstract

Cytokine mRNA expression in biopsies of Mycobacterium ulcerans-infected human tissue was investigated using real-time PCR, and the findings were correlated with the clinical stages of disease and histopathologies. A broad range of cytokine mRNAs were detected in 16 early nodules and 28 late-stage ulcers, including those for the Th1 cytokines tumor necrosis factor alpha (TNF-alpha) and gamma interferon (IFN-gamma) and the Th2 cytokine interleukin 10 (IL-10). IFN-gamma was strongly expressed in both nodules and ulcers, suggesting that a Th1 response begins early in the disease. There was a significantly higher expression of IL-8 and other proinflammatory cytokines in results from 32 biopsies with neutrophilia than in those from 12 biopsies without acute inflammation. Ten tissue samples containing granulomas showed high mRNA expression for IFN-gamma, IL-1beta, IL-12p35, IL-12p40, IL-15, and TNF-alpha relative to 34 tissue samples without granulomas. These results suggest that the human immune response to M. ulcerans is similar to that seen with some other mycobacteria despite the presence of the toxin mycolactone in the tissues.

References

Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Oct 1, 1985·Pathology·J Hayman, A McQueen
Nov 1, 1974·Archives of Dermatology·R E KriegD H Connor
Jan 15, 1997·The Journal of Clinical Investigation·D H LibratyR L Modlin
Jul 27, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·H AungE A Rich
Oct 24, 2000·International Journal of Dermatology·E Palenque
Feb 17, 2001·Infection and Immunity·T M GoodingR M Robins-Browne
Jul 27, 2001·Tropical Medicine & International Health : TM & IH·Y StienstraT S van der Werf
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 14, 2002·Infection and Immunity·Travis M GoodingRoy M Robins-Browne
Oct 16, 2002·Annals of the Rheumatic Diseases·S H E Kaufmann
Apr 10, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Travis M GoodingPaul D R Johnson
Jun 5, 2003·Emerging Infectious Diseases·Jeannette GuarnerDavid A Ashford
Jul 1, 1964·The Journal of Pathology and Bacteriology·O G DODGE
Jan 9, 1965·British Medical Journal·P D MARSDENH F LUNN
Jan 12, 2005·Clinical and Diagnostic Laboratory Immunology·B Daan WestenbrinkTjip S van der Werf
Jan 18, 2005·Current Opinion in Immunology·Alexandre AlcaïsLaurent Abel
Jan 1, 1948·The Journal of Pathology and Bacteriology·P MacCALLUM, J C TOLHURST

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Citations

Dec 17, 2008·Nature Reviews. Microbiology·Caroline DemangelStewart T Cole
May 23, 2007·The Journal of Experimental Medicine·Emmanuelle CoutanceauCaroline Demangel
Feb 10, 2009·Medical Microbiology and Immunology·Kris HuygenFrançoise Portaels
Oct 28, 2006·The Journal of Investigative Dermatology·Elisabetta PeduzziClaudia Andrea Daubenberger
Sep 29, 2007·Clinical and Experimental Immunology·H S SchipperT S van der Werf
Nov 2, 2013·Natural Product Reports·Anne-Caroline ChanyNicolas Blanchard
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rachel E SimmondsBrian M Foxwell
Dec 17, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Egídio TorradoJorge Pedrosa
Jan 10, 2012·Burns : Journal of the International Society for Burn Injuries·Rosa M SalgadoEdgar Krötzsch

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