Cytokine production in culture by cells isolated from the synovial membrane

Journal of Autoimmunity
F M BrennanM Feldmann

Abstract

Cytokine expression was investigated in synovial cell cultures isolated from diseased joints of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Tumour necrosis factor alpha (TNF alpha) was produced spontaneously at high levels by both RA and OA synovial cells. There was no correlation between cell composition and level of TNF alpha produced. In contrast, lymphotoxin (LT) and Interferon gamma (IFN gamma) (greater than 50 pg/ml) were not found. This was further investigated in the RA cultures at both the mRNA and protein level. The results indicated that high levels of mRNA were produced spontaneously by these cells, but the relevant protein was undetectable. The presence of inhibitors and/or shed receptor is unlikely to account for undetectable LT as most of the recombinant LT added to the cultures could be recovered. In addition, in some cases this resulted in an increase in IL-1 production indicating that LT is biologically active in this system. It is not clear at this stage why the protein for these mediators (produced by activated T cells) are not found in the rheumatoid cultures, although it may be due to a post-transcriptional mechanism. This possibility is currently being investigated in addition to the ...Continue Reading

Citations

Jul 11, 1998·Journal of Biomedical Materials Research·S P BendallD S Hungerford
Dec 4, 1998·Springer Seminars in Immunopathology·F M BrennanM Feldmann
Feb 23, 2002·Joint, Bone, Spine : Revue Du Rhumatisme·Marc Feldmann, Ravinder N Maini
Sep 11, 2002·Cytokine & Growth Factor Reviews·Evangelos T AndreakosMarc Feldmann
May 30, 2002·Nature Reviews. Immunology·Marc Feldmann
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R O WilliamsR N Maini
Jun 9, 2001·Thyroid : Official Journal of the American Thyroid Association·C Ruwhof, H A Drexhage
Jul 1, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M BakhietM Mustafa
Oct 10, 2009·Rheumatology·Philippa HillyerFionula M Brennan
Sep 1, 1995·Journal of Internal Medicine·L KlareskogG Holm
Sep 1, 1990·Scandinavian Journal of Immunology·L RemvigK Bendtzen
Dec 1, 1990·Scandinavian Journal of Immunology·M SvensonK Bendtzen
Feb 1, 1991·Immunological Reviews·M FeldmannK Barrett
Jan 1, 1994·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·E E KaragouniA Sklavounou
Sep 7, 1995·Annals of the New York Academy of Sciences·M FeldmannR N Maini
Jan 1, 1996·Annual Review of Immunology·M FeldmannR N Maini
Mar 1, 1993·The Journal of Clinical Investigation·G HahnG R Burmester
Nov 5, 2008·The Journal of Clinical Investigation·Fionula M Brennan, Iain B McInnes
Jan 12, 2005·Arthritis Research & Therapy·Marcus KöllerGeorg Schett
Aug 25, 2011·Arthritis Research & Therapy·Flavia Calmon-HamatyJacques Morel
Nov 29, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Mariel O HagenMiodrag Belosevic
Mar 19, 2014·Cytokine & Growth Factor Reviews·Nancy H Ruddle
Nov 29, 2013·Arthritis Research & Therapy·Constanze BuhrmannMehdi Shakibaei
Jun 16, 2012·Clinical Reviews in Allergy & Immunology·Cindy L H YangAllan S Y Lau
Jun 21, 2011·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Samuel W Hall, Anne Cooke
Jul 25, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·Hayley L EamesIrina A Udalova
Jan 23, 2009·Lancet·Lars KlareskogStephen Paget
Feb 12, 2008·The Journal of Hand Surgery·Branavan SivakumarNorbert Kang

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