PMID: 9541462Apr 16, 1998Paper

Cytokine production in PBMC from allergics and non-allergics following in vitro allergen stimulation

Immunology Letters
E LaggingB Härfast

Abstract

Dysregulation of cytokine production in atopic individuals has previously been clearly demonstrated. In the present study we aimed to assess whether a prolonged in vitro exposure of peripheral blood lymphocytes (PBMC) to allergen would result in a, by time, changed cytokine profile in allergic subjects. Blood was taken from 11 atopic asthmatic subjects and nine healthy non-atopic controls during the birch pollen season. PBMC were stimulated with birch pollen allergen (100, 1000 and 10,000 SQ-U/ml). After different times of exposure (1, 3 and 5 weeks) interleukin (IL)-5 and interferon-gamma (IFN-gamma) production was measured. Prior to the IL5 and IFN-gamma determinations, PHA was added to the cultures to ensure maximal release of cytokines. The asthmatic group always displayed a lower IFN-gamma:IL-5 ratio. Significant differences in ratio between the two groups were observed. Furthermore, PBMC challenged in vitro with 10,000 SQ-U/ml resulted in significantly elevated levels of IL-5 in the asthmatic group compared to the control group when determined after 3 and 5 weeks stimulation. In the asthmatic group the balance between IL-5 and IFN-gamma is shifted towards increased IL-5 production following prolonged in vitro stimulation....Continue Reading

References

May 1, 1991·The Journal of Clinical Investigation·Q HamidP K Jeffery
Mar 1, 1996·Immunology Today·T R Mosmann, S Sad
Nov 1, 1996·The Journal of Allergy and Clinical Immunology·J H ChangM Chan-Yeung
Dec 1, 1996·Immunology·U Döbbeling

❮ Previous
Next ❯

Citations

Sep 30, 2006·Digestive Diseases and Sciences·Kiyoshi YamazakiHirohito Kita
Feb 25, 2000·Allergy·C NilssonB Härfast
May 8, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B SachsH F Merk
Feb 7, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J M SmartA S Kemp
May 8, 2004·The Journal of Allergy and Clinical Immunology·Machteld M TiemessenEls Van Hoffen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.