Cytokine profile and nitric oxide levels in peritoneal macrophages of BALB/c mice exposed to the fucose-mannose ligand of Leishmania infantum combined with glycyrrhizin.

Parasites & Vectors
Hasan Namdar AhmadabadAshok Aspatwar

Abstract

The fucose-mannose ligand (FML) of Leishmania infantum is a complex glycoprotein which does not elicit adequate immunogenicity in humans. In recent years, adjuvant compounds derived from plants have been used for improving the immunogenicity of vaccines. Glycyrrhizin (GL) is a natural triterpenoid saponin that has known immunomodulatory activities. In the present study, we investigated the effects of co-treatment with FML and GL on the production of cytokines and nitric oxide (NO) by macrophages, in vitro. Lipopolysaccharide (LPS) stimulated murine peritoneal macrophages were treated with FML (5 μg/ml) of L. infantum and various concentrations of GL (1 μg/ml, 10 μg/ml and 20 μg/ml). After 48 h of treatment, cell culture supernatants were recovered and the levels of TNF-α, IL-10, IL-12p70 and IP-10 were measured by sandwich ELISA and NO concentration by Griess reaction. Our results indicate that the treatment of activated macrophages with FML plus GL leads to enhanced production of NO, TNF-α and IL-12p70, and reduction of IL-10 levels in comparison with FML treatment alone. Therefore, we concluded that GL can improve the immunostimulatory effect of FML on macrophages and leads to their polarization towards an M1-like phenotype.

References

Jan 1, 1996·The American Journal of Chinese Medicine·H YiK Isobe
Jan 3, 2006·Vaccine·Elvira M SaraivaClarisa Beatriz Palatnik-de-Sousa
Dec 25, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Avijit SarkarMitali Chatterjee
Sep 14, 2011·Journal of Tropical Medicine·Krystal J Evans, Lukasz Kedzierski
May 23, 2012·Frontiers in Immunology·Amrita Das, Nahid Ali
Sep 4, 2012·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Bahram BibakHasan Namdar Ahmadabad
Dec 25, 2012·Cellular Immunology·Narges BordbarZahra Amirghofran
Apr 11, 2015·Journal of Immunological Methods·Keerti Jain, N K Jain
Sep 7, 2016·Revista Da Sociedade Brasileira De Medicina Tropical·Mariana Costa DuarteEduardo Antonio Ferraz Coelho
Oct 26, 2016·The Journal of Investigative Dermatology·Takashi YamashitaShinichi Sato
Mar 21, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Qing-Ping DengRun-Yue Huang
Aug 30, 2017·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·B SarkariB Mohammadi-Ghalehbin
Dec 15, 2017·PLoS Neglected Tropical Diseases·Alicia Ponte-SucreBarbara Papadopoulou
Nov 16, 2018·Frontiers in Immunology·Fernanda Tomiotto-PellissierWander Rogério Pavanelli
May 28, 2019·Experimental Parasitology·Shafiei RezaGhatee Mohammad Amin
Jul 31, 2019·International Journal of Preventive Medicine·Mohammad MoafiRoya Taleban
Oct 23, 2019·Journal of Water and Health·Hadi MirahmadiHarlan R Barker

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Methods Mentioned

BETA
lavage
FCS
ELISA

Software Mentioned

GraphPad Prism

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