Cytokine profiles of in vivo activated thyroid-infiltrating T cells cloned in the presence or absence of interleukin 4
Abstract
We compared Th1 and Th2 cytokines secreted by randomly selected, intrathyroidal CD4+ T cell clones isolated from a patient with Graves' disease using IL-4 + IL-2 versus IL-2 alone. Prior to T cell isolation, PCR of cDNA from the intact thyroid tissue generated IL-4 and IL-10, but not IFN-gamma, products. As controls, IL-4, IFN-gamma and IL-10 cDNA was amplified from stimulated, but not unstimulated, PBMC. All 21 of the nine IL-2 clones and twelve IL-2 + IL-4 clones isolated from the thyroid tissue were CD4+. With the exception of one clone in the IL-2 group, all clones produced IL-10 on stimulation with anti-CD3 and phorbol-12-myristate 13-acetate (PMA) with similar mean values for both groups. The majority of clones in both groups also produced IFN-gamma and IL-4 after stimulation. However, the IL-4:IFN-gamma ratios were significantly higher in clones isolated using IL-2 + IL-4 than in those isolated with IL-2 alone. Furthermore, the distribution of Th1, Th0 and Th2 type clones, defined by their IL-4:IFN-gamma ratios, was also significantly different between those isolated using IL-2 alone and those isolated using IL-2 + IL-4. Of note, although Th0 clones predominated in both groups, Th1 clones were only obtained using IL-2 al...Continue Reading
References
Skin-derived aeroallergen-specific T-cell clones of Th2 phenotype in patients with atopic dermatitis
Human T-cell clones from autoimmune thyroid glands: specific recognition of autologous thyroid cells
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