Cytokine profiling for chemical sensitizers: application of the ribonuclease protection assay and effect of dose

Toxicology and Applied Pharmacology
L M PlitnickR J Smialowicz

Abstract

Exposure to chemicals in domestic and occupational settings may contribute to increases in asthma and allergy. Airway hypersensitivity (AHS) is T helper-2 (Th2) cell associated, whereas contact hypersensitivity (CHS) is T helper-1 (Th1) cell associated. The distinct cytokine profiles produced by these cells may provide a means of distinguishing respiratory sensitizers from contact sensitizers. In this study, female BALB/c mice were exposed twice on the flanks and three times on the ears using the airway sensitizer trimellitic anhydride (TMA) or the contact sensitizer dinitrochlorobenzene (DNCB). At various times following exposure, total mRNA was extracted from draining lymph node cells and cytokine mRNA profiles analyzed using a multiprobe ribonuclease protection assay (RPA). The Th2 cytokines IL4, IL10, and IL13 were significantly increased in response to TMA compared to DNCB, with optimal detection occurring 14 days following initial exposure. To determine its effect, dose was varied in flank exposures, ear exposures, or both simultaneously. When dose was varied during flank exposures only, TMA induced higher levels of Th2 cytokines than DNCB at all doses tested. DNCB did not induce Th1 cytokines at any dose tested. Variatio...Continue Reading

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Citations

Dec 2, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Darrell R BoverhofMichael R Woolhiser
Nov 26, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ian KimberRebecca J Dearman
Jul 1, 2005·Environmental Toxicology and Pharmacology·Jong Kwon LeeHye Young Oh
May 13, 2015·Toxicology·Stella A CochraneIan Kimber
Aug 12, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Catherine J BettsIan Kimber
Jan 15, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L LauensteinK Sewald
Mar 3, 2012·Toxicology and Applied Pharmacology·Michèle GoutetMasarin Ban
Aug 19, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Aimen K FarrajMaryjane Selgrade
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Dec 13, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Michael P HolsappleMichael R Woolhiser
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