Cytokine profiling of chemical allergens in mice: measurement of message versus protein

Toxicology
R J DearmanI Kimber

Abstract

Chemical respiratory allergy is an important occupational health problem, but there are currently available no validated methods for hazard identification. There has been interest for some time in the application of cytokine profiling for the characterization of chemical allergens. We have now examined whether these cytokine expression patterns are regulated at the level of mRNA and/or protein production. Mice (BALB/c strain) were exposed topically to the contact allergen 2,4-dinitrochlorobenzene (DNCB), or to the respiratory allergen trimellitic anhydride (TMA). Thirteen days after the initiation of exposure, a single cell suspension of draining (auricular) lymph node cells (LNC) was prepared. Cells were cultured for 24-120h and supernatants analyzed for cytokine protein by cytokine bead array. In parallel experiments total RNA was prepared from freshly isolated or cultured cells and cytokine gene expression was analyzed by ribonuclease protection assay (RPA) or by real time reverse transcription-polymerase chain reaction (RT-PCR). DNCB-activated LNC secreted high levels of the type 1 cytokines interferon (IFN)-gamma and IL-12 compared with TMA-stimulated LNC. The converse type 2 pattern was observed following treatment with T...Continue Reading

References

Apr 1, 1992·The Journal of Allergy and Clinical Immunology·A M BentleyA B Kay
Oct 1, 1991·Immunological Reviews·A Bendelac, R H Schwartz
Oct 1, 1991·Immunological Reviews·T R MosmannD F Fiorentino
Jan 1, 1991·International Archives of Allergy and Applied Immunology·R J DearmanI Kimber
Jan 1, 1989·The Journal of Allergy and Clinical Immunology·U Wass, L Belin
Dec 1, 1980·Contact Dermatitis·C D CalnanR J Rycroft
Aug 1, 1996·Toxicology Letters·A J Frew
Jul 1, 1997·Clinical Reviews in Allergy & Immunology·I Kimber, R J Dearman
Jul 24, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P Cullinan
Nov 20, 1998·Scandinavian Journal of Immunology·S R Paludan
Jan 5, 2000·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·C MappL M Fabbri
Apr 5, 2001·Journal of Applied Toxicology : JAT·R J Dearman, I Kimber
Jul 27, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J M MathesonM I Luster
Mar 22, 2002·Toxicology and Applied Pharmacology·L M PlitnickR J Smialowicz
Oct 2, 2002·Journal of Applied Toxicology : JAT·Rebecca J DearmanIan Kimber
Nov 7, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R J DearmanI Kimber
Feb 13, 2003·Contact Dermatitis·Ian KimberG F Gerberick
Feb 1, 1965·Archives of Dermatology·E V BUEHLER
Apr 2, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Aimen K FarrajNorbert E Kaminski
May 22, 2004·Journal of the American Academy of Dermatology·Daniel W ShawHoward I Maibach
Apr 20, 2005·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R J DearmanI Kimber
Dec 13, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Michael P HolsappleMichael R Woolhiser
Mar 9, 2006·The Journal of Allergy and Clinical Immunology·Meinir G JonesPaul Cullinan
Aug 31, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Maryjanek SelgradeStephen H Gavett
Dec 13, 2006·Methods : a Companion to Methods in Enzymology·Josje H E Arts, C Frieke Kuper
Apr 6, 2007·Biochimie·Khalid S A Khabar, Howard A Young
Nov 21, 2007·Toxicology and Applied Pharmacology·Darrell R BoverhofMichael R Woolhiser

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Citations

Jan 12, 2010·Toxicology Letters·Darrell R BoverhofMichael R Woolhiser
Nov 21, 2008·Journal of Applied Toxicology : JAT·Rebecca Jane DearmanIan Kimber
Jan 25, 2012·Journal of Applied Toxicology : JAT·R J DearmanI Kimber
Mar 16, 2011·Critical Reviews in Toxicology·Jonathan BorakMark A Pemberton

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