Cytokine profiling of pancreatic fluid using the ePFT collection method in tandem with a multiplexed microarray assay.

Journal of Immunological Methods
Joao A PauloDarwin L Conwell

Abstract

Cytokines are secreted immunomodulating proteins involved in pancreatic stellate cell activation and propagation of fibrosis in chronic pancreatitis. We aim to show that cytokines can be identified from pancreatic fluid by (1) collecting pancreatic fluid with the ePFT method, (2) processing the fluid for cytokine-targeted microarray analysis, and (3) comparing cytokine profiles in pancreatic fluid of chronic pancreatitis (CP) patients and of chronic abdominal pain (CAP) controls. We endoscopically collected pancreatic fluid from patients with CP and those with CAP using the ePFT method. This fluid was subjected directly to a multiplexed cytokine protein microarray assay. Six patients (3 CP, 3 CAP) underwent a secretin-stimulated ePFT. The mean peak bicarbonate concentrations [meq/L] of the CP and CAP patients were 43 and 97, respectively. Statistically significant decreases in the cytokine concentrations of EGF, IP-10, eotaxin, IL-3, MIP-1a, IL-15, PDGF-AB/BB, and IL-1a were observed in the CP specimens (p<0.05). We have successfully identified differences in the abundance of cytokines in ePFT-collected pancreatic fluid with a multiplexed microarray assay comparing CP and CAP controls. Further targeted investigation of cytokine...Continue Reading

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Citations

Dec 4, 2014·Analytica Chimica Acta·Julie A Stenken, Andreas J Poschenrieder
Jan 18, 2013·Gastroenterology Research and Practice·Linda S LeeJoao A Paulo
Aug 3, 2016·The American Journal of Gastroenterology·Phil A HartDarwin L Conwell
Aug 11, 2017·Pancreas·Hannah M KomarGregory B Lesinski
Jul 12, 2016·Pancreas·Karoly HorvathZhaoping He
May 28, 2019·Journal of Pediatric Gastroenterology and Nutrition·Karoly HorvathIvor D Hill
Dec 29, 2020·Pancreas·Zobeida Cruz-MonserrateUNKNOWN Consortium for the Study of Chronic Pancreatitis, Diabetes, and Pancreatic Cancer##

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