Cytokine release kinetics of concentrated growth factors in different scaffolds

Clinical Oral Investigations
Min YuJing Qiao

Abstract

Concentrated growth factors (CGF) exhibit superior potential for periodontal tissue regeneration; however, little is known about the release pattern of CGF over time. This study was designed to investigate the in vitro release pattern of growth factors and cytokines from CGF in deproteinized bovine bone mineral (DBBM) and intrafibrillarly-mineralized collagen (IMC). CGF, CGF mixed with DBBM (CGF-DBBM), and CGF mixed with IMC (CGF-IMC) were cultured in vitro for 28 days and media supernatants were collected after 24 h, 72 h, and 7, 14, 21, and 28 days respectively. The factors investigated included platelet-derived growth factor-BB, transforming growth factor beta 1, vascular endothelial growth factor, insulin-like growth factor 1, basic fibroblast growth factor, C3a, and C5a. We found that CGF-IMC released the highest total amount of cytokines compared to CGF and CGF-DBBM (p < 0.01). Growth factors were continuously released till 28 days. The release curves of most growth factors and cytokines included two peaks at 24 h and during 14 to 28 days. CGF-IMC released much more growth factors than CGF and CGF-DBBM during 14 to 28 days. Within the limitation of the study, CGF-IMC offers advantages over CGF-DBBM and CGF for sustained r...Continue Reading

References

Feb 25, 2000·Thrombosis Research·M LópezC L Arocha-Piñango
Sep 15, 2005·British Journal of Pharmacology·Anna RadomskiMarek W Radomski
Mar 22, 2008·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Duretti FufaMartha M Murray
Dec 24, 2010·Regenerative Medicine·Kerstin M Galler, Rena N D'Souza
Jul 23, 2011·Microscopy Research and Technique·Luigi Fabrizio RodellaRita Rezzani
Apr 11, 2012·Journal of Periodontal Research·A Stavropoulos, U M E Wikesjö
May 1, 2012·Nanomedicine : Nanotechnology, Biology, and Medicine·Gianni F GuidettiMauro Torti
Apr 23, 2013·Journal of Dental Research·F ChmilewskyI About
Jun 15, 2013·Immunity & Ageing : I & a·Antonino AlbaneseGiuseppina Campisi
Jan 1, 2014·Journal of Materials Science. Materials in Medicine·Anne-Marie HaaparantaMinna Kellomäki
Oct 16, 2014·Journal of Periodontology·Mark A ReynoldsMarc L Nevins
Dec 30, 2014·Transfusion Medicine Reviews·Maria Rosaria De PascaleClaudio Napoli
Jun 5, 2015·Vojnosanitetski pregled. Military-medical and pharmaceutical review·Siniga MirkovićTatjana Pugkar
Oct 16, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jehan J El-JawhariPeter V Giannoudis
Oct 29, 2015·Clinical Implant Dentistry and Related Research·Yufeng ZhangRichard J Miron
Jan 27, 2016·Clinical Oral Investigations·Eizaburo KobayashiRichard J Miron
Apr 6, 2016·Current Stem Cell Reports·Elham MasoudiAli Khademhosseini
May 20, 2016·Journal of International Society of Preventive & Community Dentistry·Amol M DoiphodeVivek Tripathi
Jul 6, 2016·Journal of Endodontics·Shuai LiuYanheng Zhou
Jul 19, 2016·The Journal of Craniofacial Surgery·Mustafa Cenk DurmuşlarSevil Altindağ Kahraman
Nov 2, 2016·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Fang WangZuolin Wang
Jan 1, 2000·Pflügers Archiv : European journal of physiology·Mišo Šabovič, Aleš Blinc
Jan 13, 2017·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Jan Simak, Silvia De Paoli
Mar 1, 2017·Journal of Biomedical Science·Manish SutharVenkatesh Ponemone
Mar 10, 2017·Platelets·Jing QiaoXiangying Ouyang
May 30, 2017·Clinical Oral Investigations·Richard J MironJoseph Choukroun
Jun 27, 2017·Annals of Plastic Surgery·Yinjia DingLijun Zheng
Aug 6, 2017·Journal of Endodontics·Charlotte JeanneauImad About
Nov 1, 2016·Journal of Biomedical Nanotechnology·Yue SunYan-Heng Zhou

❮ Previous
Next ❯

Citations

Feb 25, 2020·Journal of Biomedical Materials Research. Part a·Fahimeh TabatabaeiLobat Tayebi
Feb 29, 2020·Journal of Family Medicine and Primary Care·Mebin George MathewAfreen Kauser

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.