PMID: 8589665Oct 1, 1995Paper

Cytokine response and polymerase chain reaction study of peripheral blood mononuclear cells in infants with human cytomegalovirus infection

FEMS Immunology and Medical Microbiology
H AsanumaS Chiba

Abstract

We tried to detect human cytomegalovirus (HCMV) DNA in CD4+ and CD8+ T lymphocytes from fourteen infants with HCMV hepatitis using polymerase chain reaction (PCR) assay. HCMV was isolated from their urine and anti-HCMV IgM antibody was detected in their sera. One set of primers were designed from a region--a major immediate early (IE) gene. We detected HCMV IE DNA in the specimens obtained from six infants. HCMV IE DNA was detected from CD4+ cells in two cases and from CD8+ cells in one. In three cases, HCMV IE DNA was detected from both CD4+ and CD8+ cells. We also studied the relationship between HCMV infection and serum levels of cytokines. We determined serum levels of interleukin-4 (IL-4), tumor necrosis factor alpha (TNF-alpha) and soluble interleukin 2 receptor (sIL-2R) which were associated with the activation of T lymphocytes by enzyme immunoassay. In the acute phase of HCMV infection, titers of sIL-2R were correlated with serum levels of liver enzymes in some cases. IL-4 and TNF-alpha activities were not detected in sera. It is likely that expression of viral genome on T lymphocytes as well as activities of some cytokines are associated with active HCMV infection.

References

Oct 1, 1975·The Tohoku Journal of Experimental Medicine·S ChibaT Nakao
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Sep 1, 1991·The Journal of General Virology·J Taylor-WiedemanJ H Sinclair
Oct 7, 1989·BMJ : British Medical Journal·P StanierA S Tyms
Feb 1, 1988·Epidemiology and Infection·P D Griffiths, J E Grundy
Nov 1, 1986·Pediatric Research·Y YanaseT Kawasaki
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G P RiceM B Oldstone
Jan 29, 1994·Lancet·W D DöckeH D Volk
Feb 1, 1994·The Journal of Clinical Investigation·L J GeistG W Hunninghake

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Citations

Apr 1, 1997·The Journal of Pediatrics·K NumazakiS Chiba
Dec 24, 1997·Clinical and Diagnostic Virology·K Numazaki, S Chiba
Oct 18, 2003·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Tomoko FujikawaHiroyuki Tsutsumi
Aug 10, 2019·Frontiers in Genetics·Efe SezginCheryl A Winkler
Jun 1, 1997·FEMS Immunology and Medical Microbiology·K Numazaki

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