Cytokine signalling in rat pulp interstitial fluid and transcapillary fluid exchange during lipopolysaccharide-induced acute inflammation

The Journal of Physiology
Athanasia BletsaHelge Wiig

Abstract

The dental pulp consists of loose connective tissue encased in rigid dentinal walls. Because of its topography the tissue has low interstitial compliance and limited capacity to expand during fluid volume changes. Due to limitations regarding access to interstitial fluid, basic knowledge on transcapillary fluid transport parameters is lacking for this organ. The scope of this project was dual: first we aimed at establishing a method for isolation of pulp interstitial fluid (IF), and second we applied the method in rats subjected to lipopolysaccharide (LPS)-induced endotoxaemia. The aim was to measure colloid osmotic pressure (COP) and pro-inflammatory cytokines in the pulp IF during acute inflammation. Fluid volumes and pulpal blood flow (PBF) were measured to obtain more information about microcirculatory changes that take place in this pulpitis model. By centrifugation of incisor pulp at 239 g we were able to extract fluid representative for IF. Pulp IF had a relative high control COP (approximately 83% of plasma COP) and was similar to plasma COP 3 h after LPS challenge. The pulp exhibited a high content of IF (0.60 +/- 0.03 ml (g wet weight)(-1)) and a vascular volume of 0.03 +/- 0.01 ml (g w.w.)(-1) No differences were obs...Continue Reading

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Citations

Aug 26, 2014·Journal of Endodontics·Shuichi HashimotoKatsuhisa Sunada
Apr 5, 2014·Journal of Endodontics·John S ColomboRena N D'Souza
Apr 14, 2011·The Journal of Physiology·Helge Wiig
Nov 11, 2008·Archives of Oral Biology·J L BainR B Johnson
Sep 25, 2007·Cytokine·Virve PääkkönenLeo Tjäderhane
Jul 23, 2011·The European Journal of Neuroscience·Phoebe E ScotlandArmen N Akopian
Sep 21, 2011·Archives of Oral Biology·Saulo GeraldeliKim A Brogden
Oct 17, 2015·Journal of Dental Research·E RenardB Alliot-Licht
Jan 20, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Bengt Age Sørby BorgeHelge Wiig
Aug 15, 2020·European Journal of Oral Sciences·Ellen BerggreenAnca Virtej
Apr 4, 2006·The Journal of Physiology·Lucy F Donaldson
Nov 8, 2020·Clinical Oral Investigations·Poliana Amanda Oliveira SilvaTaia Maria Berto Rezende
Apr 8, 2021·Lasers in Medical Science·Jéssica Fernanda Sena BonviciniAna Paula Turrioni
Aug 11, 2021·International Endodontic Journal·José Luis Muñoz-CarrilloOscar Gutiérrez-Coronado
Dec 8, 2021·Journal of Orofacial Orthopedics = Fortschritte Der Kieferorthopädie : Organ/official Journal Deutsche Gesellschaft Für Kieferorthopädie·Isabel KnaupMichael Wolf

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