Cytokines derived from activated human mononuclear cells markedly stimulate transferrin secretion by cultured Sertoli cells

Endocrinology
E HoebenG Verhoeven

Abstract

There is considerable evidence that Sertoli cell function is controlled not only by hormones, but also by locally produced growth factors and cytokines. To gain more insight into the nature and effects of cytokines potentially involved in the control of Sertoli cell function, we incubated rat Sertoli cells with media conditioned by activated human peripheral blood mononuclear cells. Such media (PBMC-CM) are known to be an extremely rich source of a variety of cytokines. It was demonstrated that PMBC-CM and protein fractions derived from them stimulate Sertoli cell transferrin secretion and messenger RNA production more potently then peritubular cell-conditioned medium or FIRT (a combination of FSH, insulin, retinol, and testosterone). Transferrin secretion expressed per mg cell DNA was stimulated approximately 5-fold by peritubular cell-conditioned medium or FIRT and nearly 20-fold by PBMC-CM. The effects of PBMC-CM were accompanied by a limited increase in cAMP and a noticeable rise in cGMP. Affinity chromatography on a column coated with an antiserum directed against interleukin-1 beta (IL-1 beta) showed that part of the activity in the PBMC-CM was related to IL-1 beta. The remainder of the activity was largely retained by an...Continue Reading

Citations

Feb 19, 2002·Molecular and Cellular Endocrinology·C PetersenO Söder
Mar 19, 2004·Journal of Reproductive Immunology·Cecilia PetersenOlle Söder
Sep 27, 2012·Cellular and Molecular Life Sciences : CMLS·Marco G AlvesPedro F Oliveira
Apr 25, 2000·Biology of Reproduction·D ZeyseM Huleihel
Jan 18, 2011·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Mahmoud Abu ElheijaMahmoud Huleihel
Apr 27, 2012·Physiological Reviews·Jon M Oatley, Ralph L Brinster
Mar 20, 2002·American Journal of Reproductive Immunology : AJRI·Mahmoud HuleihelMoshe Mazor
Jun 1, 1997·Transplantation Proceedings·P R SanbergD F Cameron

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