Cytokines induce tight junction disassembly in airway cells via an EGFR-dependent MAPK/ERK1/2-pathway

Laboratory Investigation; a Journal of Technical Methods and Pathology
Loredana PetecchiaGiovanni A Rossi

Abstract

Epithelial barrier permeability is altered in inflammatory respiratory disorders by a variety of noxious agents through modifications of the epithelial cell structure that possibly involve tight junction (TJ) organization. To evaluate in vitro whether pro-inflammatory cytokines involved in the pathogenesis of respiratory disorders could alter TJ organization and epithelial barrier integrity, and to characterize the signal transduction pathway involved Calu-3 airway epithelial cells were exposed to TNF-a, IL-4 and IFN-g to assess changes in: (a) TJ assembly, that is, occludin and zonula occludens (ZO)-1 expression and localization, evaluated by confocal microscopy; (b) apoptotic activity, quantified using terminal transferase deoxyuridine triphosphate nick-end labeling staining; (c) epithelial barrier integrity, detected as transmembrane electrical resistance and expressed as G(T) values; (d) epidermal growth factor receptor (EGFR)-dependent mitogenactivated protein (MAP) kinase (MAPK)/extracellular signal-regulated kinases (ERK)1/2 phosphorylation, assessed by western blotting. Exposure to cytokines for 48 h induced a noticeable downregulation of the TJ transmembrane proteins. The degree ZO-1 and occludin colocalization was 62±...Continue Reading

References

Jul 1, 1977·Journal of the National Cancer Institute·J FoghT Orfeo
Feb 1, 1995·Canadian Journal of Physiology and Pharmacology·M P SparrowH W Mitchell
Feb 1, 1994·The Journal of Membrane Biology·A G HowarthB R Stevenson
Dec 1, 1993·The Journal of Cell Biology·M FuruseS Tsukita
Jun 17, 1999·Trends in Cell Biology·S Tsukita, M Furuse
Feb 23, 2000·The Journal of Cell Biology·D Li, R J Mrsny
Mar 7, 2000·Mediators of Inflammation·J E Losa GarcíaJ L Arellano
Jul 18, 2000·American Journal of Physiology. Cell Physiology·C G KevilJ S Alexander
Aug 1, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·L L MiticJ M Anderson
Dec 5, 2000·Molecular Biology of the Cell·M SaitouS Tsukita
Mar 10, 2001·Nature·L Chang, M Karin
Nov 8, 2001·American Journal of Physiology. Cell Physiology·M AhdiehA Youakim
May 22, 2002·Cellular Signalling·Neus AgellPriam Villalonga
Sep 11, 2002·Molecular Biology of the Cell·Carolyn B CoyneLarry G Johnson
Feb 10, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Thomas Y MaHamid M Said
Apr 13, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Maria Iris HermannsCharles James Kirkpatrick
Apr 28, 2004·The International Journal of Biochemistry & Cell Biology·Nicole S Harhaj, David A Antonetti
Jun 11, 2004·Biophysical Journal·Sylvain V CostesStephen Lockett
Aug 17, 2004·Trends in Cell Biology·David M Bryant, Jennifer L Stow
Feb 11, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Thomas Y MaHamid M Said
Jun 1, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Matthias BruewerAsma Nusrat
Aug 10, 2005·Cell Biology International·Anne-Jacqueline RelovaGodfried M Roomans
Dec 7, 2006·The American Journal of Pathology·Jerrold R Turner
May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alicia L HumlicekDwight C Look
Jun 5, 2007·Current Opinion in Gastroenterology·Joachim Mankertz, Jörg-Dieter Schulzke
Aug 19, 2008·Biochimica Et Biophysica Acta·Ben N G Giepmans, Sven C D van Ijzendoorn
Sep 3, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Christine PohlCharles James Kirkpatrick
Sep 12, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·I B KibangouD Bouglé
Oct 24, 2008·Nature Reviews. Molecular Cell Biology·David M Bryant, Keith E Mostov
Oct 28, 2008·Biochimica Et Biophysica Acta·Christopher T Capaldo, Asma Nusrat
Dec 23, 2008·Archives of Biochemistry and Biophysics·Nels OlsonAlbert van der Vliet
Mar 10, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Paola D VermeerJoseph Zabner
Aug 6, 2009·The Journal of Biological Chemistry·Michael ScharlDeclan F McCole
Dec 15, 2010·Current Medicinal Chemistry·R-M CataliotoS Giuliani
Mar 1, 1993·Journal of Microscopy·E M M MandersJ A Aten

❮ Previous
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Citations

Mar 27, 2013·Molecular and Cellular Biology·Geneviève CoulombeNathalie Rivard
Jan 24, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ko-Jen LiChia-Li Yu
Mar 24, 2016·Critical Reviews in Food Science and Nutrition·Guan YangMei-Jun Zhu
Oct 10, 2015·Tissue Barriers·Cornelia BlumeDonna E Davies
Apr 11, 2015·Frontiers in Cellular and Infection Microbiology·Qinghua YuQian Yang
Apr 2, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Shadabul HaqueLisa M Kaminskas
Dec 7, 2016·Pflügers Archiv : European journal of physiology·Oliver H Wittekindt
Mar 7, 2018·International Urology and Nephrology·C WasénD Giglio
Nov 22, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nicola F FletcherJane A McKeating
Jun 7, 2018·American Journal of Physiology. Cell Physiology·Sahar BilalKurt Amsler
Oct 21, 2016·Clinical and Translational Gastroenterology·Julia KönigRobert-Jan Brummer
Feb 16, 2013·Cell Biology International·Huiling GuoYifei Huang
May 16, 2014·Current Opinion in HIV and AIDS·Peter W Hunt
Jun 27, 2014·Molecular Biology Reports·Ru-Yi LuYan-Jun Hu
Dec 22, 2015·Digestive Diseases and Sciences·Farokh R DemehriDaniel H Teitelbaum
Feb 1, 2018·Science Signaling·Rosemary PikeMaddy Parsons
Feb 23, 2020·Pediatric Pulmonology·Giovanni A RossiAndrew A Colin
Feb 27, 2020·Expert Review of Respiratory Medicine·Corrado PelaiaGirolamo Pelaia
Jun 8, 2019·International Journal of Molecular Medicine·Ruixue YangXia Zhao
Sep 22, 2017·American Journal of Respiratory Cell and Molecular Biology·Mahyar AghapourIrene H Heijink
Jun 27, 2020·The Journal of International Medical Research·Jie Yang
Feb 1, 2014·Bioscience Reports·Jin TongVictor P Kolosov
Jan 23, 2018·Frontiers in Immunology·Jürg HamacherUz Stammberger
Aug 8, 2018·Nutrients·Leila Abdelhamid, Xin M Luo
May 16, 2020·Frontiers in Immunology·Andreas FreyMichael Wegmann
Apr 23, 2015·In Vitro Cellular & Developmental Biology. Animal·Zheng ZhaoZhaozhu Li
Oct 2, 2019·Bioengineering & Translational Medicine·Chloe S KimPamela K Kreeger
May 4, 2020·Digestive Diseases and Sciences·Hoyul LeeJung-Ae Kim

❮ Previous
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