Cytokinetic furrowing in toroidal, binucleate and anucleate cells in C. elegans embryos

Journal of Cell Science
Jalal K BaruniGeorge von Dassow

Abstract

Classical experimental studies on echinoderm zygotes concluded that the juxtaposition of two astral microtubule arrays localizes the stimulus for cytokinetic furrowing. However, recent experimental and genetic studies in Caenorhabditis elegans, Drosophila and mammalian cultured cells implicate microtubules of the central spindle, and regulatory proteins associated with this structure, suggesting that the essential conditions for furrow induction may differ from one animal cell to another. We used micromanipulation and laser microsurgery to create, in three ways, the juxtaposition of astral microtubules in C. elegans embryonic cells. In toroidal cells we observe that furrows initiate both where astral microtubule arrays are juxtaposed, and where the cortex most closely approaches the central spindle. We find that binucleate cells successfully furrow not only across the spindles, but also between unconnected spindle poles. Finally, we find that anucleate cells containing only a pair of centrosomes nevertheless attempt to cleave. Therefore, in C. elegans embryonic cells, as in echinoderms, juxtaposition of two asters suffices to induce furrowing, and neither the chromatin nor the physical structure of the central spindle are indis...Continue Reading

References

May 1, 1974·Genetics·S Brenner
Mar 21, 1983·Journal of Theoretical Biology·J G White, G G Borisy
Jan 1, 1995·Methods in Cell Biology·L G Edgar
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·C L RiederG Sluder
Oct 21, 1998·Current Biology : CB·J PowersW Saxton
Nov 6, 1998·Nature·L Timmons, A Fire
May 17, 2002·Current Biology : CB·Charles B Shuster, David R Burgess
Oct 1, 1961·The Journal of Experimental Zoology·R RAPPAPORT
Dec 26, 2003·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Thomas D Pollard
Jul 7, 2005·The Journal of Cell Biology·William M BementGeorge von Dassow
Aug 5, 2005·Nature·Henrik Bringmann, Anthony A Hyman
Aug 17, 2005·The Journal of Cell Biology·Ozlem YüceMichael Glotzer
Sep 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sara O DeanJames A Spudich
Dec 15, 2005·Journal of Cell Science·Yukako Nishimura, Shigenobu Yonemura
Oct 13, 2006·Journal of Cell Science·Pier Paolo D'AvinoDavid M Glover
Dec 27, 2006·Current Biology : CB·Henrik BringmannAnthony A Hyman

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Citations

Jun 27, 2008·Molecular Biology of the Cell·Rosalind V Silverman-GavrilaAndrew Wilde
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Edwin Munro, Bruce Bowerman
Dec 17, 2009·The Journal of Cell Biology·George von DassowWilliam M Bement
Oct 29, 2008·The Journal of Cell Biology·Victoria E Foe, George von Dassow
Jan 12, 2010·Arthropod Structure & Development·Andrew FooteDavid Merritt
Mar 17, 2009·Trends in Cell Biology·George von Dassow
Mar 12, 2016·Seminars in Cell & Developmental Biology·Masanori Mishima
Apr 2, 2009·Journal of Microscopy·I RaabeI M Tolić-Nørrelykke
Aug 28, 2015·Molecular Biology of the Cell·Christine M FieldTimothy J Mitchison
Jun 5, 2020·The Journal of Cell Biology·Bernardo Chapa-Y-LazoMasanori Mishima

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