Cytokinin- and auxin-induced stomatal opening involves a decrease in levels of hydrogen peroxide in guard cells of Vicia faba

Functional Plant Biology : FPB
Xi-Gui SongTu-Sheng Song

Abstract

Previous studies have shown that cytokinins and auxins can induce the opening of stomata. However, the mechanism of stomatal opening caused by cytokinins and auxins remains unclear. The purpose of this paper is to investigate the relationship between hydrogen peroxide (H2O2) levels in guard cells and stomatal opening induced by cytokinins and auxins in Vicia faba. By means of stomatal bioassay and laser-scanning confocal microscopy, we provide evidence that cytokinins and auxins reduced the levels of H2O2 in guard cells and induced stomatal opening in darkness. Additionally, cytokinins not only reduced exogenous H2O2 levels in guard cells caused by exposure to light, but also abolished H2O2 that had been generated during a dark period, and promoted stomatal opening, as did ascorbic acid (ASA, an important reducing substrate for H2O2 removal). However, unlike cytokinins, auxins did not reduce exogenous H2O2, did not abolish H2O2 that had been generated in the dark, and therefore did not promote reopening of stoma induced to close in the dark. The above-mentioned effects of auxins were similar to that of diphenylene iodonium (DPI, an inhibitor of the H2O2-generating enzyme NADPH oxidase). Taken together our results indicate that ...Continue Reading

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Citations

Nov 22, 2008·Plant, Cell & Environment·Geert PottersMarcel A K Jansen
Dec 20, 2008·Journal of Integrative Plant Biology·Xiao-Ping She, Xi-Gui Song
Mar 30, 2011·Plant, Cell & Environment·Vanesa B TognettiFrank Van Breusegem
Jun 5, 2015·Frontiers in Plant Science·Biswapriya B MisraSixue Chen
Aug 21, 2013·Journal of Experimental Botany·Sasan Aliniaeifard, Uulke van Meeteren
Nov 5, 2016·Journal of Experimental Botany·Mika FarberDavid Weiss
Aug 10, 2017·Frontiers in Molecular Biosciences·Sisi GengSixue Chen
Nov 26, 2008·Plant Molecular Biology·Biswa R Acharya, Sarah M Assmann
Jan 6, 2009·Physiologia Plantarum·Iva SnyrychováEva Hideg
Nov 6, 2013·The New Phytologist·Yuwei SongChun-Peng Song
Apr 2, 2021·Plant Physiology·Maren Müller, Sergi Munné-Bosch
Feb 12, 2013·The Plant Cell·Takahiro JoudoiSumio Iwai
Aug 28, 2021·International Journal of Molecular Sciences·Tibor JandaÉva Darkó
Sep 21, 2021·The FEBS Journal·Joanna KacprzykPaul F McCabe

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