Cytokinin, auxin, and abscisic acid affects sucrose metabolism conduce to de novo shoot organogenesis in rice (Oryza sativa L.) callus

Botanical Studies
Shiang-Ting Lee, Wen-Lii Huang


Shoot regeneration frequency in rice callus is still low and highly diverse among rice cultivars. This study aimed to investigate the association of plant hormone signaling and sucrose uptake and metabolism in rice during callus induction and early shoot organogenesis. The immatured seeds of two rice cultivars, Ai-Nan-Tsao 39 (ANT39) and Tainan 11 (TN11) are used in this study. Callus formation is earlier, callus fresh weight is higher, but water content is significant lower in ANT39 than in TN11 while their explants are inoculated on callus induction medium (CIM). Besides, the regeneration frequency is prominently higher in ANT39 (~80%) compared to TN11 callus (0%). Levels of glucose, sucrose, and starch are all significant higher in ANT39 than in TN11 either at callus induction or early shoot organogenesis stage. Moreover, high expression levels of Cell wall-bound invertase 1, Sucrose transporter 1 (OsSUT1) and OsSUT2 are detected in ANT39 at the fourth-day in CIM but it cannot be detected in TN11 until the tenth-day. It suggested that ANT39 has higher callus growth rate and shoot regeneration ability may cause from higher activity of sucrose uptake and metabolism. As well, the expression levels of ORYZA SATIVA RESPONSE REGUL...Continue Reading


Mar 1, 1997·The Plant Journal : for Cell and Molecular Biology·R Ehness, T Roitsch
Feb 27, 1999·Current Opinion in Plant Biology·M Sugiyama
Jan 1, 2003·Journal of Experimental Botany·T RoitschA K Sinha
Mar 29, 2003·Trends in Plant Science·Patricia León, Jen Sheen
Jun 29, 2005·Journal of Molecular Evolution·Xuemei JiJohn Bennett
Oct 18, 2005·The Plant Cell·Michael G MasonG Eric Schaller
Jan 21, 2006·Science·Jian XuBen Scheres
Oct 18, 2006·Genes & Development·Michael SauerEva Benková
Nov 10, 2006·Trends in Plant Science·Dale R Walters, Neil McRoberts
Dec 1, 2006·Plant Biotechnology Journal·Nigel G Halford, Matthew J Paul
Aug 31, 2007·Journal of Experimental Botany·Graham N ScofieldRobert T Furbank
Apr 23, 1965·Science·F SkoogC O Miller
Oct 6, 2007·Science·Bruno Müller, Jen Sheen
Aug 21, 2008·Journal of Integrative Plant Biology·Yong-Qin WangZhen Zhu
Feb 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Markéta PernisováJan Hejátko
Mar 24, 2009·Cell·Steffen Vanneste, Jirí Friml
May 21, 2009·The Plant Journal : for Cell and Molecular Biology·Ying H SuXian S Zhang
Jun 26, 2010·Nature·Zhong ZhaoJan U Lohmann
Nov 26, 2010·Plant & Cell Physiology·Ming-Der ShihYue-Ie C Hsing
Nov 29, 2011·Plant Science : an International Journal of Experimental Plant Biology·Mohammad R SiahpooshJoachim Kopka
Mar 3, 2012·Plant Physiology·Yu-Chang TsaiJoseph J Kieber

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