Cytokinin inhibits the proteasome-mediated degradation of carbonylated proteins in Arabidopsis leaves

Plant & Cell Physiology
Vanita JainSteven C Huber

Abstract

Under normal conditions, plants contain numerous carbonylated proteins, which are thought to be indicative of oxidative stress damage. Conditions that promote formation of reactive oxygen species (ROS) enhance protein carbonylation, and protein degradation is required to reverse the damage. However, it is not clear how the degradation of carbonylated proteins is controlled in planta. In this report, we show that detached Arabidopsis leaves rapidly and selectively degrade carbonylated proteins when kept in the dark. The loss of carbonylated proteins corresponded to a loss of soluble protein and accumulation of free amino acids. Degradation of carbonylated proteins and the loss of soluble protein was blocked by MG132 but not 3-methyladenine, suggesting that the 26S proteasome pathway rather than the autophagic pathway was involved. Consistent with this, rpn10 and rpn12 mutants, which are defective in proteasome function, had increased (rather than decreased) levels of carbonylated proteins when detached in the dark. Feeding metabolites (amino acids and sucrose) to detached leaves of wild-type Arabidopsis in the dark had little or no effect on the loss of carbonylated proteins, whereas providing soybean xylem sap via the transpira...Continue Reading

Citations

Feb 26, 2009·Antioxidants & Redox Signaling·Christine H Foyer, Graham Noctor
Jul 29, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Kuaifei XiaMingyong Zhang
Apr 2, 2014·Journal of Experimental Botany·Liliana Avila-OspinaCéline Masclaux-Daubresse
Feb 19, 2013·Plant Science : an International Journal of Experimental Plant Biology·Giovanna Serino, Elah Pick
Jun 11, 2015·Antioxidants & Redox Signaling·Pavel KerchevFrank Van Breusegem
Jan 17, 2012·Physiologia Plantarum·Irma N RobertsChristiane Funk
Sep 20, 2015·Molecular Plant·Tatjana M HildebrandtHans-Peter Braun

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