Cytokinin stimulates chloroplast transcription in detached barley leaves.

Plant Physiology
Yan O ZuboThomas Börner

Abstract

Chloroplasts are among the main targets of cytokinin action in the plant cell. We report here on the activation of transcription by cytokinin as detected by run-on assays with chloroplasts isolated from apical parts of first leaves detached from 9-d-old barley (Hordeum vulgare) seedlings and incubated for 3 h on a 2.2 x 10(-5) m solution of benzyladenine (BA). Northern-blot analysis also detected a BA-induced increase in the accumulation of chloroplast mRNAs. A prerequisite for BA activation of chloroplast transcription was preincubation of leaves for 24 h on water in the light, resulting in a decreased chloroplast transcription and a drastic accumulation of abscisic acid. Cytokinin enhanced the transcription of several chloroplast genes above the initial level measured before BA treatment, and in the case of rrn16 and petD even before preincubation. Cytokinin effects on basal (youngest), middle, and apical (oldest) segments of primary leaves detached from plants of different ages revealed an age dependence of chloroplast gene response to BA. BA-induced stimulation of transcription of rrn16, rrn23, rps4, rps16, rbcL, atpB, and ndhC required light during the period of preincubation and was further enhanced by light during the in...Continue Reading

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Citations

Dec 19, 2012·Biochemistry. Biokhimii︠a︡·A M AvalbaevF M Shakirova
Jun 3, 2010·Doklady. Biochemistry and Biophysics·V V KusnetsovA I Konovalov
Oct 1, 2013·Journal of Experimental Botany·Maria V YamburenkoThomas Börner
Jan 31, 2016·Journal of Experimental Botany·Sai Krishna TallaRajeshwari Ramanan
Sep 11, 2009·Current Opinion in Plant Biology·Tomás Werner, Thomas Schmülling
Apr 16, 2015·Journal of Experimental Botany·Anne Cortleven, Thomas Schmülling
May 30, 2015·Journal of Experimental Botany·Marketa ZdarskaJan Hejátko
Feb 15, 2015·Biochimica Et Biophysica Acta·Thomas BörnerVictor V Kusnetsov
Oct 21, 2016·Plant Physiology and Biochemistry : PPB·Farida ShakirovaAzamat Avalbaev
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Dec 19, 2018·International Journal of Molecular Sciences·Martin HönigKarel Doležal
May 11, 2018·Plant, Cell & Environment·Helena JanečkováMartina Špundová
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Aug 15, 2017·The Plant Journal : for Cell and Molecular Biology·Jörg MeurerNikolay Manavski
Apr 16, 2021·Frontiers in Plant Science·Yoshihisa IkedaMitsuhiro Aida

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