PMID: 13549496May 25, 1958Paper

Cytological and chemical studies of the growth of protoplasts of Bacillus megaterium

The Journal of Biophysical and Biochemical Cytology
P C FITZ-JAMES

Abstract

Aeration of protoplasts of Bacillus megaterium in a succinate buffered nutrient broth led to marked growth similar to that already described by McQuillen, and the degree of chromatin synthesis in these growing forms prompted a combined cytological and chemical study. Growth was followed by phase contrast and by Feulgen stains, as well as by lipide phosphorus, nucleic acid, and protein analyses. In slide cultures, growth and compression led to monstrous flattened forms with readily visible, but coalescent nuclear structures. In fluid cultures, the protoplasts grew as phase dense spheres. Orderly reproduction of apparently discrete nuclear bodies was observed during the initial hours of spherical growth, but in older cultures, the chromatin arrangement tended to be more haphazard and was influenced by the concentration of Mg ions. In the same medium, protoplasts free of lysis showed a linear rise in optical density, while vegetative cells exhibited an exponential increase. However, protoplasts were able to synthesize DNA at the same rate as vegetative cells, but their increase of RNA was always less. Thus, as they grew, the ratio RNA/DNA fell. The lipide P increased in proportion to the expanding surface. With growth and lysis, l...Continue Reading

References

Nov 1, 1955·Biochimica Et Biophysica Acta·K MCQUILLEN

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Citations

Nov 1, 1955·Biochimica Et Biophysica Acta·K MCQUILLEN
Aug 1, 1965·The Journal of Cell Biology·P Fitz-James, R Hancock
Dec 1, 1977·Journal of Dental Research·A C Haller, M L Zimny
Oct 1, 1963·Journal of Cellular Physiology·P L KUEMPEL, A B PARDEE
Sep 1, 1973·The American Journal of Anatomy·M L Zimny
Jan 1, 1971·Zeitschrift für Zellforschung und mikroskopische Anatomie·M L Zimny, E D Levy
Sep 14, 2019·Future Medicinal Chemistry·Anne K BuckLouise Eo Darling
Jan 1, 1975·Journal of Bacteriology·K FodorL Alföldi
Jun 1, 1961·Bacteriological Reviews·M R SALTON
Dec 1, 1975·Bacteriological Reviews·J W Greenawalt, T L Whiteside
May 1, 1960·Journal of Bacteriology·C WEIBULL, H BECKMAN
Jun 1, 1960·Journal of Bacteriology·M J PINE
Mar 1, 1971·Journal of Bacteriology·G S RothL Daneo-Moore
Mar 1, 1971·Journal of Bacteriology·P C Fitz-James

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