Cytological and molecular characterizations of a novel 2A nullisomic line derived from a widely-grown wheat cultivar Zhoumai 18 conferring male sterility

PeerJ
Zhixin JiaoJi-Shan Niu

Abstract

A dwarf, multi-pistil and male sterile dms mutant was previously reported by us. However, the genetic changes in this dms are unclear. To examine the genetic changes, single nucleotide polymorphism (SNP) association, chromosome counting, and high-resolution chromosome fluorescence in situ hybridization (FISH) techniques were employed. By comparing tall plants (T) with dwarf plants (D) in the offspring of dms mutant plants, SNP association analysis indicated that most SNPs were on chromosome 2A. There were three types in offspring of dms plants, with 42, 41 and 40 chromosomes respectively. High-resolution chromosome painting analysis demonstrated that T plants had all 42 wheat chromosomes; the medium plants (M) had 41 chromosomes, lacking one chromosome 2A; while D plants had 40 wheat chromosomes, and lacked both 2A chromosomes. These data demonstrated that dms resulted from a loss of chromosome 2A. We identified 23 genes on chromosome 2A which might be involved in the development of stamens or pollen grains. These results lay a solid foundation for further analysis of the molecular mechanisms of wheat male sterility. Because D plants can be used as a female parent to cross with other wheat genotypes, dms is a unique germplasm f...Continue Reading

References

Oct 1, 1993·The Plant Cell·R B GoldbergP M Sanders
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Shaoxing HuangPiotr Gornicki
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·K. GuptaP. Leroy
Feb 19, 2003·Functional & Integrative Genomics·Lili QiBikram S Gill
Apr 3, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·C GroosG Charmet
Apr 26, 2003·The Plant Journal : for Cell and Molecular Biology·Barbara DespresMartine Devic
Oct 19, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Daryl J SomersKeith Edwards
Dec 9, 2004·The Plant Journal : for Cell and Molecular Biology·Jeremy PrestonRoger W Parish
Jan 19, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Q J SongP B Cregan
Jul 20, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·James BealesDavid A Laurie
Jan 16, 2009·The Plant Journal : for Cell and Molecular Biology·Yan ZhangSheila McCormick
Sep 9, 2009·Development·Aurélien Boisson-DernierUeli Grossniklaus
Dec 25, 2009·Sexual Plant Reproduction·Anna A DobritsaRobert J Swanson
Feb 12, 2010·Cold Spring Harbor Protocols·Joseph D Clarke
Oct 12, 2010·Plant Signaling & Behavior·Hui Li, Dabing Zhang
Nov 26, 2010·Nature·Li-Qing ChenWolf B Frommer
Oct 5, 2012·BMC Genomics·Stefan K SchulzeAndreas S Beutler
Jan 10, 2013·Genome Research·Jonathon T HillH Joseph Yost
Nov 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Huakun ZhangBao Liu
Feb 11, 2014·Current Opinion in Plant Biology·Dabing Zhang, Li Yang
Dec 20, 2014·Plant Physiology·Christelle LerouxJean-Claude Mollet
Feb 11, 2015·Plant Physiology·Simon PearceZoe A Wilson
Apr 6, 2017·The Plant Journal : for Cell and Molecular Biology·Ze LiuWanqi Liang
May 13, 2017·Nature Communications·Chuan XiaXiuying Kong
Oct 17, 2017·The Plant Journal : for Cell and Molecular Biology·Hong LinZoe A Wilson
Nov 8, 2017·Proceedings of the National Academy of Sciences of the United States of America·Zheng WangLigeng Ma
Mar 13, 2018·Frontiers in Plant Science·Richard G BrowneRoger W Parish

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-seq
PCRs
genotyping
PCR
GTPases
nucleotide exchange

Software Mentioned

Tophat2
R
BMKCloud
GATK
HaplotypeCaller
Hem I
SPSS
DESeq R
Circos

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.