Cytological and morphological analysis of hybrids between Brassicoraphanus, and Brassica napus for introgression of clubroot resistant trait into Brassica napus L

PloS One
Zongxiang ZhanChunyu Zhang

Abstract

Interspecific hybridization is a powerful tool for improvement of crop species, it has the potential to broaden the genetic base and create new plant forms for breeding programs. Synthetic allopolyploid is a widely-used model for the study of genetic recombination and fixed heterosis in Brassica. In Brassica napus breeding, identification and introgression of new sources of clubroot resistance trait from wild or related species into it by hybridization is a long-term crop management strategy for clubroot disease. Radish (Raphanus sativus L.) is a close relative of the Brassica and most radish accessions are immune to the clubroot disease. A synthesized allotetraploid Brassicoraphanus (RRCC, 2n = 36) between R. sativus cv. HQ-04 (2n = 18, RR) and Brassica oleracea var. alboglabra (L.H Bailey) (2n = 18, CC) proved resistant of multiple clubroot disease pathogen P. brassicae. To predict the possibility to transfer the clubroot resistance trait from the RR subgenome of allotetraploid Brassicoraphanus (RRCC, 2n = 36) into Brassica napus (AACC, 2n = 38), we analyzed the frequency of chromosome pairings in the F1 hybrids produced from a cross between B. napus cv. HS5 and the allotetraploid, characterize the genomic composition of some...Continue Reading

References

Nov 11, 1995·Nucleic Acids Research·P VosM Kuiper
Sep 5, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·K SuwabeM Hirai
Dec 23, 2003·Evolution; International Journal of Organic Evolution·Richard CronnJonathan F Wendel
Feb 27, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·A ChandraS S Banga
Jul 15, 2004·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Maoteng LiZongyun Li
Sep 20, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M LeflonA M Chèvre
Feb 14, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Lili QiBikram S Gill
Apr 12, 2007·Genome Génome / Conseil National De Recherches Canada·C J SchelfhoutJ M Wroth
Aug 19, 2007·Science·Loren H Rieseberg, John H Willis
Jul 4, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Young-Pyo LeeSunggil Kim
Jul 10, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Koji SakamotoEtsuo Matsumoto
Apr 17, 2009·Genome Génome / Conseil National De Recherches Canada·Jiuhuan FengSteven S Xu
Dec 17, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Akito KameiMasashi Hirai
Sep 13, 2012·The New Phytologist·Donald A Levin
Jul 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Z LiP Luo
Sep 1, 1992·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M HagimoriH Yoshikawa
Jul 6, 2014·Breeding Science·Hiroshi Yamagishi, Shripad R Bhat

❮ Previous
Next ❯

Citations

Jan 7, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Qinfei LiHongyuan Song
May 26, 2021·Plant Biotechnology Journal·Dandan HuJun Zou

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

ogura
R
INRA
NTSYS Clustering

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.