PMID: 9554582Apr 29, 1998Paper

Cytological comparison of leaves and stems of Prunus avium L. shoots cultured on a solid medium with agar or gelrite

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
T FranckT Gaspar

Abstract

An axillary proliferating clone of Prunus avium L. was subcultured every four weeks on solid MS medium with agar as the gelling agent. Vitrification (hyperhydricity) of shoots was induced in one four week cycle with the same medium except that agar was replaced by gelrite. During culture on the vitrifying medium, the water content of the shoots progressively increased with a parallel decrease in chlorophyll content. Cytological differences between the leaves and stems of the vitrified and normal shoots were detected by light and electron (both transmission and scanning) microscopy. Leaves of vitrified shoots were characterized by lower number of chloroplasts in the palisade parenchyma and by a defective cuticle. The stems of vitrified shoots had a less developed and lignifled xylem tissue, lacked sclerenchymatic areas and showed hypertrophy of the cortical parenchyma. More intense vacuolar activity with evaginations of the chloroplast envelope into the vacuole was noted in cells of vitrified leaves.

Citations

Apr 12, 2005·Plant Physiology and Biochemistry : PPB·Shady SaherEnrique Olmos
Jul 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·D RogerE Lainé
May 5, 2001·Plant Science : an International Journal of Experimental Plant Biology·T FranckJ F. Hausman
Oct 15, 2013·Journal of Experimental Botany·Niels van den DriesGeert-Jan M de Klerk
Jul 13, 2004·Plant Physiology and Biochemistry : PPB·Thierry FranckGinette Deby-Dupont
Mar 23, 2004·Physiologia Plantarum·Shady SaherEnrique Olmos
Apr 28, 1999·FEBS Letters·V N Luzikov

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