PMID: 13563546Jul 25, 1958Paper

Cytological effects of prefixation treatment

The Journal of Biophysical and Biochemical Cytology
D A HUNGERFORD, M DIBERARDINO

Abstract

The effects produced by prefixation treatments on cells in metaphase from 10-day mouse fetuses and from several embryonic stages of the frog were investigated. The technical value of some of these pretreatments is noted. Pretreatment with isotonic solutions (both ionic and non-ionic in the case of the mouse, ionic only in the frog) generally produced a similar effect, viz., chromosomal swelling with little effect on the spindle. A notable exception is provided by frog embryos preceding the neurula stage; spindle disorganization without chromosomal swelling was produced by pretreatment in isotonic modified Niu-Twitty solution, containing no divalent cations. Pretreatment with hypotonic solutions (both ionic and non-ionic in the case of the mouse, ionic only in the frog) generally produced several major effects, viz., despiralization of chromosomes, chromatid separation, and spindle disorganization. The conclusion is drawn from the mouse data that, in order to produce these effects, pretreating solutions must be of low osmotic pressure. Low ionic strength alone (e.g., isotonic sucrose solutions) is not sufficient. As differentiation of frog embryos progressed, pretreatments either of longer duration or with solutions of increasin...Continue Reading

References

Nov 1, 1956·Stain Technology·C E FORD, J L HAMERTON
Jan 1, 1956·Cold Spring Harbor Symposia on Quantitative Biology·T J KING, R BRIGGS
Sep 1, 1953·Proceedings of the National Academy of Sciences of the United States of America·M C Niu, V C Twitty
Jun 1, 1954·Proceedings of the National Academy of Sciences of the United States of America·D Mazia
Mar 15, 1955·Proceedings of the National Academy of Sciences of the United States of America·D Steffensen
May 15, 1955·Proceedings of the National Academy of Sciences of the United States of America·T J King, R Briggs
Aug 1, 1956·Proceedings of the National Academy of Sciences of the United States of America·S Wolff, H E Luippold
Mar 15, 1957·Proceedings of the National Academy of Sciences of the United States of America·B P Kaufmann, M R McDonald

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Citations

Jan 1, 1969·Zeitschrift für Zellforschung und mikroskopische Anatomie·R G McKinnellL A Reed
Feb 1, 1962·Developmental Biology·J SIGNORETR R HUMPHREY
Nov 1, 1967·Developmental Biology·R A FlockingerP J Stambrook
Dec 1, 1969·Developmental Biology·J K Reynhout, D L Kimmel
Oct 1, 1970·The Journal of Experimental Zoology·S Dasgupta
May 1, 1970·The Journal of Experimental Zoology·P J Stambrook, R A Flickinger
Nov 1, 1962·The Journal of Experimental Zoology·S DASGUPTA
Dec 1, 1962·The Journal of Experimental Zoology·E P VOLPE, S DASGUPTA
Jan 1, 1967·Irish Journal of Medical Science·N P Bishun
Apr 1, 1969·Psychological Reports·L W West, H W Zingle
Nov 15, 1961·Klinische Wochenschrift·W MASSHOFF, E SCHLEIERMACHER
Jan 1, 1965·Chromosoma·J Signoret
Jun 1, 1976·Perceptual and Motor Skills·L S Walker, P H Wright
Jul 25, 1969·Science·R G McKinnellD D Labat

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