Cytolysis of human erythrocytes by a covalent antibody-selenium immunoconjugate

Free Radical Biology & Medicine
L Chen, J E Spallholz

Abstract

A selenium (Se)-containing immunoconjugate of a human anti-erythrocyte membrane antibody (Ab-Se) has been synthesized via oxidation of the carbohydrate moieties of the antibody and covalent coupling with selenocystamine. The isolated Ab-Se immunoconjugate is shown to be more hemolytic than is selenocystamine when expressed on equivalent selenium basis. Native antibody preincubated with the human erythrocytes prevented hemolysis induced by the Ab-Se immunoconjugate. As observed microscopically, the Ab-Se immunoconjugate caused extensive damage to the erythrocyte membrane and lysis. The cytotoxicity of Se toward the human erythrocytes is believed to be caused initially by the localized generation of superoxide (O2.-) within the cell membrane. This is the first demonstration of site-directed immunoselectivity of Se cytotoxicity and demonstrates the potential for a free radical pharmacology based on localized Se-generated O2.-.

References

Mar 23, 1978·The New England Journal of Medicine·B M Babior
Mar 30, 1978·The New England Journal of Medicine·B M Babior
Apr 1, 1992·Biological Trace Element Research·G N Schrauzer
Jul 1, 1992·Archives of Biochemistry and Biophysics·J ChaudiereJ Leclaire
Mar 1, 1991·Molecular Microbiology·A BöckF Zinoni
May 20, 1987·Journal of Immunological Methods·D J O'Shannessy, R H Quarles
May 1, 1987·Journal of Medical Virology·E BiziagosR Deloince
Mar 1, 1969·Archives of Biochemistry and Biophysics·R C Dickson, A L Tappel
Aug 1, 1969·The Journal of Nutrition·H H Draper, A S Csallany
Mar 15, 1983·Biochemical Pharmacology·M L Hu, J E Spallholz
Sep 1, 1980·Clinical Toxicology·C G Wilber
Nov 1, 1993·Biological Trace Element Research·C MacVicar, G D Frenkel
Jul 1, 1994·Free Radical Biology & Medicine·J E Spallholz
Feb 1, 1994·Experimental Eye Research·K Hightower, J McCready
Apr 1, 1994·Cancer Letters·Y Gong, G D Frenkel
Oct 1, 1993·Free Radical Biology & Medicine·K Faulkner, I Fridovich

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.