PMID: 6971753Jun 1, 1980Paper

Cytolytic activity of pulmonary and systemic lymphoid cells from C57BL/6 mice following intrapulmonary or intraperitoneal immunization with allogeneic tumor cells

Experimental Lung Research
J L Caldwell, H B Kaltreider

Abstract

In order to demonstrate whether specific cytotoxic T cells could be induced in lung parenchyma, C57BL/6 mice were immunized by the intrapulmonary route with allogenetic tumor cells (P815). Ten days after administration of 20 x 10(6) allogeneic cells, peak concentrations of cytotoxic cells were found in lung, tracheobronchial lymph node, and spleen. With reduction in immunizing dose, lytic activity disappeared from spleen and lymph node, but persisted in lung. The cytolytic activity was specific for the immunizing alloantigen, was abolished by antitheta serum, and could not be attributed to macrophages. For comparison, C57BL/6 mice were immunized by the intraperitoneal route with 20 x 10(6) P815 cells. The expected cytolytic activity was found in spleen and lymph nodes: however, unexpectedly high levels of cytolytic activity were also found in pulmonary lymphocytes. This activity was confirmed using a wide range of effector to largest cell ratios in the assay system. Quantitative cytolytic assays demonstrated that the maximum rate of cytolysis by pulmonary lymphocytes obtained from mice immunized intraperitoneally exceeded by 10- to 20-fold the rate of cytolysis by pulmonary lymphocytes obtained from mice receiving intrapulmonar...Continue Reading

References

Jan 1, 1978·Scandinavian Journal of Immunology·K L Yap, G L Ada
Nov 1, 1978·The Journal of Experimental Medicine·F A EnnisP Albrecht
Nov 4, 1976·The New England Journal of Medicine·M NewhouseJ Bienenstock
May 1, 1976·Infection and Immunity·G H Scott, J S Walker
Jan 1, 1974·Advances in Immunology·J C Cerottini, K T Brunner
Jan 1, 1974·International Archives of Allergy and Applied Immunology·W R ThomasD Keast

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Citations

Jan 1, 1985·Annals of the New York Academy of Sciences·D D Joel, A D Chanana
Dec 1, 1983·Infection and Immunity·M F LipscombJ Stein-Streilein

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