Cytolytic capabilities of lamina propria and intraepithelial lymphocytes in normal and chronically inflamed human intestine

Scandinavian Journal of Immunology
Silvia MelgarM-L Hammarström

Abstract

Cell-mediated lymphocyte cytotoxicity in ileum and colon of patients with ulcerative colitis (UC), Crohn's disease (CD) and controls was investigated. Frequencies of cells expressing perforin and Fas-ligand (FasL) were determined by immunomorphometry. mRNA expression of perforin, granzyme B and FasL in T cells and subsets was assayed by reverse transcriptase-polymerase chain reaction. Cytotoxicity of intraepithelial and lamina propria lymphocytes was analysed without ex vivo activation in three functional assays: (1) anti-CD3-dependent T-cell receptor (TCR)-/CD3-mediated redirected cytotoxicity, (2) Fas-/FasL-mediated TCR-/CD3-independent cytotoxicity and (3) natural killer (NK) cell cytotoxicity. Inflammation in ileum of CD patients caused increased frequency of perforin-expressing cells and enhanced perforin-dependent TCR-/CD3-mediated cytotoxicity. In contrast, lymphocytes in the inflamed colon of UC or Crohn's colitis patients did not display this cytotoxicity nor did lymphocytes of normal colon. Normal colon lymphocytes showed spontaneous Fas-/FasL-mediated cytotoxicity. This activity was retained but not enhanced in inflamed UC colon. In contrast, a significant increase of FasL-expressing cells was seen in situ. Inflammat...Continue Reading

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Citations

Sep 29, 2009·Inflammatory Bowel Diseases·Maximilian J WaldnerMarkus F Neurath
Jul 31, 2007·International Immunology·Göte ForsbergMarie-Louise Hammarström
Apr 17, 2013·Biochemistry. Biokhimii︠a︡·T S Zamolodchikova
Dec 2, 2008·Clinical and Experimental Immunology·S HirosakoH Kohrogi
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Sep 17, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Tuomo S Kokkonen, Tuomo J Karttunen

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