Cytomegalovirus initiates infection selectively from high-level β1 integrin-expressing cells in the brain

The American Journal of Pathology
Hideya KawasakiToshihide Iwashita

Abstract

Cytomegalovirus (CMV) is a prevalent pathogen in intrauterine infections that causes congenital anomalies such as CMV encephalitis, which is characterized by the focal areas of reactive gliosis, reactive mononuclear cells, microglial nodules, and ventriculoencephalitis. To elucidate the mechanisms of CMV susceptibility in the developing brain, cell tropism and the infectious dynamics of CMV infection were investigated. We evaluated intraventricular and intravascular infections from the perspective of the distribution of CMV and its receptor (β1 integrin) in the earliest phase of infection. Murine CMV (MCMV) immediate early 1-positive cells were colocalized mainly with meninges and choroid plexus (after intraventricular infection) or with endothelial cells and pericytes (after intravascular infection). Using green fluorescent protein-expressing recombinant MCMV particles and fluorescent microbeads (100 to 300 nm), we revealed that CMV particle size is the primary factor determining the initial CMV distribution. β1 Integrin inhibition using a shRNA and functional blocking antibody significantly reduced MCMV infection. IHC analysis, flow cytometric, and brain slice analyses strongly support that high-level β1 integrin-expressing c...Continue Reading

References

Mar 5, 1992·The New England Journal of Medicine·K B FowlerC A Alford
Jan 1, 1992·Archives of Virology·M N WykesG R Shellam
Jan 1, 1992·Current Eye Research·M F BeersmaT E Feltkamp
Jul 1, 1984·Journal of Virology·K Ebihara, Y Minamishima
Nov 1, 1993·Journal of Virology·C SöderbergE Möller
Feb 1, 1994·The Journal of Clinical Investigation·L J GeistG W Hunninghake
Aug 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D Shepro, N M Morel
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y TomookaT Sakakura
Dec 1, 1996·Journal of Neurosurgery·B ZünkelerE H Oldfield
Mar 18, 1999·The American Journal of Pathology·S Aiba-MasagoY Tsutsui
Jul 28, 1999·The Journal of General Virology·R Pietropaolo, T Compton
Sep 27, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·I KosugiY Tsutsui
Oct 16, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hideya KawasakiYoshihiro Tsutsui
Jun 7, 2003·Brain Research. Brain Research Reviews·Andrzej W Vorbrodt, Danuta H Dobrogowska
Oct 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Adam L FeireTeresa Compton
Apr 19, 2005·Nature Medicine·Xin WangEng-Shang Huang
Jun 22, 2005·BMC Public Health·Michael J Cannon, Katherine Finn Davis
Aug 16, 2005·Reviews in Medical Virology·Yoshihiro TsutsuiHideya Kawasaki
Feb 14, 2006·British Medical Bulletin·David R Chadwick
Apr 13, 2007·Journal of Virology·Marisa K IsaacsonTeresa Compton
Feb 13, 2008·The Journal of Experimental Medicine·Thad KoontzWilliam J Britt
May 14, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Li LiYoshihiro Tsutsui
Jul 18, 2008·Journal of Virology·Penghua WangErol Fikrig
Dec 18, 2008·The Journal of General Virology·Kimberly M HsuWayne M Yokoyama
Jan 13, 2009·Clinical Microbiology Reviews·Maxim C-J CheeranMark R Schleiss
Mar 24, 2009·Journal of Neurovirology·Nathan S IveyAndrew A Lackner
Jun 6, 2009·Congenital Anomalies·Yoshihiro Tsutsui
Aug 19, 2009·PLoS Biology·Karine LoulierCharles Ffrench-Constant

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Citations

Apr 4, 2016·Cell Stem Cell·Tomasz J NowakowskiArnold R Kriegstein
Sep 9, 2015·Frontiers in Bioengineering and Biotechnology·Christian Claude LachaudBernat Soria
Dec 2, 2017·FEBS Letters·Gulistan AgirmanLaurent Nguyen
Aug 31, 2019·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hideya KawasakiTakahiko Hariyama
Oct 13, 2018·Nature Reviews. Neuroscience·John V ForresterSamantha J Dando
Oct 27, 2017·American Journal of Physiology. Cell Physiology·Alexa N LauerChristian Schwerk
Mar 5, 2020·Molecular Biology Reports·Francesca RombiSharon Yeoh
Mar 13, 2021·Reviews in Medical Virology·Krishneel SinghWilliam D Rawlinson
Jul 10, 2021·Molecular Immunology·Kate J DixonSubramaniam Malarkannan

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