PMID: 8955410Dec 1, 1996Paper

Cytometric detection of mycobacterial surface antigens: exposure of mannosyl epitopes and of the arabinan segment of arabinomannans

Journal of Bacteriology
V OzanneM Daffé

Abstract

The physical arrangement of cell envelope components leads to the exposure of selected structural motifs which in turn may influence host-parasite interactions. To gain insight into the exposed epitopes, the present study describes a flow cytometric method designed to probe defined molecules on dispersed mycobacteria. The hydrophobic fluorophore N-hexadecanoyl aminofluorescein inserted in the mycobacterial cell envelope permitted focusing of fluorescence-activated cell sorter analysis on cells that were further labeled with defined monoclonal antibodies and fluorochrome-coupled streptavidin. The use of antibodies directed against the lipooligosaccharide of Mycobacterium tuberculosis demonstrated the specific detection of the antigen on the cell surface of a Canetti-like strain of M. tuberculosis, and not on those of mycobacterial strains that were devoid of the glycolipid. Thus, the method was applied to investigate the relative amounts of surface-exposed mannosylated compounds and D-arabinan-containing substances of different strains of the tubercle bacillus and a strain of the rapidly growing nonpathogenic species Mycobacterium smegmatis. Both M. tuberculosis and M. smegmatis are endowed with mannosyl and arabinan epitopes on...Continue Reading

References

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Citations

Jun 4, 2005·Journal of Industrial Microbiology & Biotechnology·Shu-Chi ChangPeter Adriaens
Jan 1, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bartek RajwaJ Paul Robinson
Jan 30, 2008·Cytometry. Part B, Clinical Cytometry·Howard M Shapiro, Nancy G Perlmutter
Nov 12, 2010·Clinical and Vaccine Immunology : CVI·Jae-Sung YuBarton F Haynes
Mar 27, 2010·FEMS Microbiology Reviews·Susann Müller, Gerhard Nebe-von-Caron
Jul 31, 2013·Orvosi hetilap·Eva Pállinger
Mar 26, 2004·Nature Reviews. Microbiology·Didier RaoultMichel Drancourt
Jul 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jordi B TorrellesLarry S Schlesinger
Sep 5, 2008·Current Protocols in Cytometry·Kristi R Harkins, Kelley Harrigan
Feb 3, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·M Daffé, G Etienne
Feb 7, 2001·Clinical Microbiology Reviews·A Alvarez-BarrientosM Sánchez-Pérez

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