PMID: 3753191Jul 1, 1986Paper

Cytomorphological changes in liver cells exposed to allyl and benzyl isothiocyanate and their cysteine and glutathione conjugates

Archives of Toxicology
J H TemminkP J van Bladeren

Abstract

Since allyl isothiocyanate has been reported to be a bladder carcinogen and benzyl isothiocyanate is a known anti-carcinogen, it is important to know the mode of their cytotoxic action. This was investigated in a RL-4 hepatocyte cell line by studying the morphological effects of increasing concentrations of the isothiocyanates and their glutathione and cysteine conjugates. These effects were compared with those induced by tert-butylhydroperoxide which supposedly has its primary effect upon the cytosolic glutathione status and thus upon the integrity of Ca2+-sequestrating mitochondria. The results agree with the previously postulated role of conjugation in the exposure of cells to isothiocyanates: Conjugates show effects similar to those produced by the free parent compounds because conjugates release free isothiocyanates in aqueous solution. The cytomorphological effects increase in a more or less dose-dependent manner with increasing concentrations of isothiocyanate or exposure time. Probably due to increased exposure, suspended RL-4 cells are more sensitive to the toxic action than cells growing on a substrate. No qualitative differences were found between the effects of allyl and benzyl isothiocyanate, indicating that their ...Continue Reading

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Citations

Jan 1, 1990·Chemico-biological Interactions·R M Vos, P J Van Bladeren
Jan 1, 1988·Toxicology in Vitro : an International Journal Published in Association with BIBRA·I M BruggemanP J van Bladeren
Jan 1, 1989·Toxicology in Vitro : an International Journal Published in Association with BIBRA·I M BruggemanP J van Bladeren
Nov 19, 2003·Life Sciences·Adebowale AdebiyiR N V Prasad
Mar 5, 2004·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Adebowale AdebiyiR N V Prasad
Sep 24, 2015·Journal of Food Science and Technology·Israel Sunmola AfolabiSolomon Oladapo Rotimi
Jan 1, 1988·Die Nahrung·H J LewerenzR Macholz
Jan 1, 1992·Die Nahrung·H J LewerenzR Plass
Aug 1, 1988·Trends in Pharmacological Sciences·P J van Bladeren
Jan 1, 1987·Critical Reviews in Oncology/hematology·M A Lea

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