PMID: 2108243Mar 1, 1990Paper

Cytopathic action of Naegleria fowleri amoebae on rat neuroblastoma target cells

The Journal of protozoology
F Marciano-CabralS G Bradley

Abstract

The axenically cultured, weakly pathogenic Naegleria fowleri LEE and the highly pathogenic, mouse passaged N. fowleri LEEmp are cytopathic for B103 rat nerve cells in culture. Cytopathogenicity was measured by release of radiolabeled rubidium or radiolabeled chromium from B103 target cells. Cytopathogenicity was time-dependent for up to 18 h and dependent upon amoebae effector to nerve cell target ratios of less than 1:1. Release of 51Cr from B103 cells by either LEE or LEEmp amoebae was enhanced by addition of calcium or magnesium to medium free of these divalent cations but the ion-channel inhibitor, verapamil, or the ionophore A23187 and phorbol myristate acetate did not alter release of 51Cr from B103 cells cocultured with the amoebae. Cycloheximide or actinomycin D impaired release of 51Cr from B103 target cells injured by either LEE or LEEmp amoebae. Both strains of amoebae were fractionated by glass bead disruption and high speed centrifugation into membrane and soluble fractions. Each fraction was incubated with either 86Rb or 51Cr labeled nerve cells. The membrane fraction from LEEmp was more active than the soluble fraction in facilitating rubidium and chromium release. In contrast, the soluble fraction from LEE was m...Continue Reading

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Citations

Feb 1, 1995·International Journal for Parasitology·P R BadenochD J Coster
Apr 25, 2007·Immunology Letters·Julie GertnerJean-Jacques Fournié
Apr 23, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Attila Tárnok
Jul 29, 2004·Molecular and Biochemical Parasitology·Seok-Ryoul JeongHo-Joon Shin
Apr 12, 2002·The Journal of Biological Chemistry·Rosa HerbstMatthias Leippe
May 9, 2000·Clinical and Diagnostic Laboratory Immunology·H J ShinK I Im
May 1, 1993·FEMS Microbiology Letters·P L FioriP Cappuccinelli
Feb 7, 2018·PLoS Biology·Frances MercerPatricia J Johnson
Nov 1, 1991·Infection and Immunity·W N HuW J Kopachik
Apr 1, 1994·Infection and Immunity·H AlizadehJ Y Niederkorn

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