PMID: 19912864Jun 1, 1992Paper

Cytoplasmic and nuclear vasopressin RNA in hypothalamic and extrahypothalamic neurons of the Brattleboro rat: An in situ hybridization study

Molecular and Cellular Neurosciences
P Szot, D M Dorsa

Abstract

In situ hybridization was performed in Long-Evans (LE) and heterozygous (HET) and homozygous (HOM) Brattleboro rats using a 48-base oligonucleotide complementary to the last 16 amino acids of the vasopressin messenger RNA (VP mRNA). The number of cells expressing the VP mRNA in the supraoptic (SON) and paraventicular (PVN) nuclei was not significantly different between LE, HET, and HOM rats; however, the relative amount of mutant VP mRNA expressed in neurons of the PVN and SON of the HOM rat was significantly lower than that in the other two genotypes. In contrast, the suprachiasmatic nucleus (SCN), the bed nucleus of the stria terminalis (BNST), and the medial amygdala (MA) of the HOM rat showed a significant reduction in both the number of neurons and the level of mutant VP mRNA expression per cell compared to those in either the LE or the HET rat. To determine whether the reduced level of mutant VP mRNA in the HOM rat was due to decreased transcription, in situ hybridization to detect the VP primary transcript was performed. The number of neurons and the amount of nuclear VP RNA expressed per cell for the PVN, SCN, BNST, and MA in the HET and HOM rats were not significantly different from those in the LE rat. However, in the...Continue Reading

References

Oct 1, 1986·Physiology & Behavior·J P Kroon, A L Riley
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G R UhlJ F Habener
Mar 8, 1985·The Journal of Comparative Neurology·G J DeVriesD F Swaab
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R Ivell, D Richter
Jan 1, 1983·Cell and Tissue Research·F van Leeuwen, R Caffé
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J A MajzoubJ F Habener

❮ Previous
Next ❯

Citations

Dec 27, 1995·European Journal of Pharmacology·P PoulinQ J Pittman
Dec 1, 1994·Brain Research. Molecular Brain Research·M M ShoresR C Veith
Jul 1, 1994·Brain Research. Molecular Brain Research·P SzotD M Dorsa
Nov 5, 1997·Biological Psychiatry·J R StevensP Szot
Dec 1, 1996·Epilepsy Research·J R StevensP Szot
Jan 29, 1993·Brain Research·D P FiglewiczD M Dorsa
Nov 24, 1999·Frontiers in Neuroendocrinology·B J GeddesJ B Uney

❮ Previous
Next ❯

Related Concepts

Related Feeds

Amygdala: Sensory Processes

Amygdalae, nuclei clusters located in the temporal lobe of the brain, play a role in memory, emotional responses, and decision-making. Here is the latest research on sensory processes in the amygdala.

Amygdala and Midbrain Dopamine

The midbrain dopamine system is widely studied for its involvement in emotional and motivational behavior. Some of these neurons receive information from the amygdala and project throughout the cortex. When the circuit and transmission of dopamine is disrupted symptoms may present. Here is the latest research on the amygdala and midbrain dopamine.