Cytoplasmic and nucleic calcium oscillations in immature mouse oocytes: evidence of wave polarization by confocal imaging

Experimental Cell Research
B LefèvreJ Testart

Abstract

Using confocal laser scanning microscopy and time-lapse reconstruction, we monitored [Ca2+]i in living mouse oocytes loaded with the calcium-sensitive fluorescent dyes fluo-3/AM or NuCa Green (specific for nuclear calcium) at the time of meiosis reinitiation. Our data confirm that spontaneous Ca2+ oscillations occurred in most of the immature fully grown mouse oocytes at least during the first half hour after release of the follicle and these oscillating oocytes underwent GVB in a large proportion after 1 h of culture. These spontaneous oscillations were disrupted when GVB was inhibited by dbcAMP and suppressed in the absence of external calcium. They were dependent on intracellular InsP3-sensitive Ca2+ stores since they were inhibited by heparin, an InsP3-receptor antagonist, or thapsigargin, which depletes InsP3-sensitive Ca2+ stores. A relation appeared between the germinal vesicle chromatin appearance and the oocyte's ability to exhibit calcium oscillations. NuCa Green-loaded oocytes exhibited clear fluorescence oscillations in the nuclear region which lead us to discuss the implication of the nuclear calcium in the meiotic process. Moreover, the observation of a clear polarization of the Ca2+ waves in 30% of the oocytes pe...Continue Reading

Citations

Aug 1, 1997·Zygote : the Biology of Gametes and Early Embryos·J PetrF Jílek
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·R PeyM Hafner
Oct 30, 2016·Journal of Cellular Physiology·Meenakshi TiwariShail K Chaube
Oct 7, 2008·Reproductive Sciences·Arlette PestyBrigitte Lefèvre
Feb 1, 1997·Molecular Reproduction and Development·H WuR A Fissore
Jul 11, 2007·Molecular Reproduction and Development·F MiyaraB Lefèvre
Jan 4, 2001·Molecular Reproduction and Development·J PetrZ Nemecek
Oct 31, 2006·Molecular Human Reproduction·A PestyC Poirot
Mar 26, 1998·Biochemical and Biophysical Research Communications·L Santella
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Aug 2, 2005·Cell Calcium·Juan Claudio BenechJosé Roberto Sotelo
Aug 17, 2005·Cell Calcium·Oleg Gerasimenko, Alexei Tepikin

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