PMID: 9168140Apr 26, 1997Paper

Cytoplasmic delivery of calcein mediated by liposomes modified with a pH-sensitive poly(ethylene glycol) derivative

Biochimica Et Biophysica Acta
Kenji KonoT Takagishi

Abstract

Previously, as a new type of pH-sensitive liposome, we prepared egg yolk phosphatidylcholine (EYPC) liposomes bearing succinylated poly(glycidol), that is a poly(ethylene glycol) derivative having carboxyl groups, and showed that fusion ability of the liposomes increases under weakly acidic and acidic conditions (Kono, K., Zenitani, K. and Takagishi, T. (1994) Biochim. Biophys. Acta 1193, 1-9). In this study, we examined intracellular delivery of a water-soluble molecule, calcein, mediated by the succinylated poly(glycidol)-modified liposomes. When CV-1 cells, an established line of African green monkey kidney cells, were incubated with bare EYPC liposomes containing calcein at 37 degrees C, only weak and vesicular fluorescence of calcein was observed by using a fluorescence microscope. In contrast, the cells treated with the polymer-modified liposomes containing calcein displayed more intensive and diffuse fluorescence, indicating that calcein was transferred into the cytoplasm. Uptake of the polymer-modified liposomes by the cells was shown to decrease slightly as amount of the polymer fixed on the liposome increases. However, the fluorescence of calcein observed in the liposome-treated cell was, on the contrary, enhanced as ...Continue Reading

References

Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S Ohkuma, B Poole
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J BentzF C Szoka
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C J GallowayI Mellman
Nov 2, 1994·Biochimica Et Biophysica Acta·C PuyalJ Philippot

❮ Previous
Next ❯

Citations

Apr 11, 2000·Lipids·F Van BambekeM P Mingeot-Leclercq
Apr 7, 2004·Advanced Drug Delivery Reviews·Elias FattalCatherine Dubernet
Apr 7, 2004·Advanced Drug Delivery Reviews·Sérgio SimõesMaria C Pedroso de Lima
Apr 7, 2004·Advanced Drug Delivery Reviews·Marie-Andrée Yessine, Jean-Christophe Leroux
Jul 3, 1999·Chemistry and Physics of Lipids·J A Boomer, D H Thompson
Sep 29, 2001·Biomaterials·E TouitouF Levi-Schaffer
Aug 5, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·K KonoT Takagishi
Aug 23, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Junhwa ShinDavid H Thompson
Dec 18, 2001·Advanced Drug Delivery Reviews·K Kono
Jun 6, 2000·Advanced Drug Delivery Reviews·E MastrobattistaG Storm
Mar 4, 2000·Advanced Drug Delivery Reviews·C J Provoda, K D Lee
Apr 17, 2002·International Journal of Pharmaceutics·Toshiro MizoueFuminori Moriyasu
Aug 15, 2002·International Journal of Pharmaceutics·Emmanuelle RouxJean-Christophe Leroux
Nov 29, 2012·Nature Communications·Eijiro MiyakoYoshihisa Hagihara
Nov 30, 2010·Therapeutic Delivery·Mohanad MossalamCarol S Lim
Sep 13, 2013·Therapeutic Delivery·Diego Dos Santos FerreiraMônica Cristina Oliveira
Jun 6, 2000·Advanced Drug Delivery Reviews·O V GerasimovD H Thompson
Jan 20, 2016·Journal of Liposome Research·Shivani Rai PaliwalSuresh Prasad Vyas
Jan 6, 2016·Journal of Agricultural and Food Chemistry·Md NuruzzamanRavi Naidu
Sep 27, 2008·Expert Opinion on Drug Delivery·Malavosklish Bikram, Jennifer L West
Nov 10, 2005·Journal of Drug Targeting·Kapil KhatriSuresh P Vyas
Dec 31, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ulrich HuthRegine Peschka-Süss
Jan 17, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Faina KarchemskiOren Regev
Oct 15, 2011·Advanced Drug Delivery Reviews·Arnaud E FelberJean-Christophe Leroux
Nov 22, 2015·Biochimica Et Biophysica Acta·Eugénia NogueiraArtur Cavaco-Paulo
Mar 9, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eiji YubaKenji Kono
Jul 6, 2007·Biomaterials·Azadeh Kheirolomoom, Katherine W Ferrara
May 22, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ban-An KhawWilliam C Hartner
Apr 14, 2007·The Journal of Pharmacy and Pharmacology·H Karanth, R S R Murthy
Dec 21, 2005·International Journal of Pharmaceutics·Tatsuhiro IshidaHiroshi Kiwada
Jun 13, 2014·Pharmaceutical Biology·Saeed GhanbarzadehSanam Arami
Dec 14, 2011·Journal of Liposome Research·Zeljka VanićRolf Schubert
Feb 7, 2002·The Journal of Pharmacy and Pharmacology·Myo-Sook HongChong-Kook Kim
Jul 2, 2016·Pharmacological Reviews·Phatsapong YingchoncharoenDes R Richardson
Jul 21, 2007·International Journal of Pharmaceutics·Ryo SuzukiKazuo Maruyama
Feb 10, 2017·Drug Delivery·Iordana NeamtuAurica P Chiriac

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.