Cytoplasmic expression of pontin in renal cell carcinoma correlates with tumor invasion, metastasis and patients' survival

PloS One
Xiang ZhangZhonghua Xu

Abstract

Renal cell carcinoma (RCC) is the most lethal of all genitourinary malignancies. Distant metastasis represents the major cause of death in patients with RCC. Recent studies have implicated the AAA+ ATPase pontin in many cellular activities that are highly relevant to carcinogenesis. In this study, we demonstrate for the first time that pontin was up-regulated in RCC, and plays a previously unknown pro-invasive role in the metastatic progression of RCC through epithelial-to-mesenchymal transition (EMT) pathway. 28 pairs of freshly frozen clear cell RCC samples and the matched normal renal tissues analyzed by quantitative RT-PCR and western blotting demonstrated that pontin was up-regulated in clear cell RCC tissues than in normal renal tissues. In addition, immunohistochemistry was used to evaluate subcellular pontin expression in 95 RCC patients, and found that overexpression of pontin in cytoplasm positively correlated with the metastatic features, predicting unfavorable outcomes of RCC patients. Furthermore, in vitro experiments show pontin was predominantly expressed in cytoplasm of RCC cell lines, and a significant suppression of cell migration and invasion in pontin siRNA treated RCC cell lines was observed. Mechanistic st...Continue Reading

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Citations

Oct 8, 2013·PloS One·Armin WiegeringChristoph-Thomas Germer
Dec 29, 2015·International Journal of Molecular Medicine·Eva JuengelRoman A Blaheta
Sep 9, 2017·Frontiers in Molecular Biosciences·Yu-Qian Mao, Walid A Houry
Jun 21, 2018·Journal of Cellular Biochemistry·Chunming YangFandong Meng

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Methods Mentioned

BETA
xenografts
PCR
reverse transcription-PCR
transfection
nuclear translocation

Software Mentioned

SPSS

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