Cytoplasmic free calcium mobilization in platelets, expression of P-selectin, phosphatidylserine, and microparticle formation, measured by whole blood flow cytometry, in hypertensive patients. Effect of doxazosin GITS

Thrombosis Research
Manuel LabiósJusto Aznar

Abstract

The effects of doxazosin on expression of CD62 (P-selectin) and phosphatidylserine on platelet membrane and platelet calcium flux were studied in 50 uncomplicated essential hypertensive patients (World Health Organization stages 1-2) and 80 normotensive control subjects, matched for age, sex, and cardiovascular risk factors. Hypertensive patients showed greater in vivo platelet activation at baseline than control patients (percentage of CD62-positive platelets, 4.1+/-2.2% versus 2.4+/-1.5%, p<0.001; percentage of phosphatidylserine-positive platelets, 0.8+/-0.5% versus 0.5+/-0.3%, p<0.001). Increased platelet activation was associated with significant changes in the mobilization of free intraplatelet calcium, evaluated by a whole blood flow cytometric kinetic method. With this method, an arbitrary Ca(2+) mobilization index was defined as the ratio of cytoplasmic free calcium before activation with thrombin to the slope of the calcium removal rate following the action of the agonist. This index was significantly higher in untreated hypertensive patients than in normotensive controls (0.12+/-0.06 versus 0.05+/-0.08, p<0.001). Treatment of hypertensive patients with doxazosin gastrointestinal therapeutic system (4 mg/day as a sing...Continue Reading

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Citations

May 23, 2015·Mediators of Inflammation·Elzinandes Leal de AzeredoLuzia Maria de-Oliveira Pinto
Oct 2, 2012·Biochemical Pharmacology·Pasquale PignatelliFrancesco Violi
Jan 23, 2010·American Journal of Hypertension·Eugenia GkaliagkousiAlbert Ferro
Nov 13, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Pasquale PignatelliFrancesco Violi
May 23, 2012·Circulation·Pasquale PignatelliFrancesco Violi
Dec 17, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Olivier MorelFlorence Toti
Feb 5, 2019·American Journal of Cardiovascular Drugs : Drugs, Devices, and Other Interventions·Annabella Braschi
Jul 18, 2015·Journal of Immunology Research·Christoph FickentscherMartin Herrmann

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