Cytoplasmic Heme-Binding Protein (HutX) from Vibrio cholerae Is an Intracellular Heme Transport Protein for the Heme-Degrading Enzyme, HutZ

Biochemistry
Yukari SekineTakeshi Uchida

Abstract

HutZ is a cytoplasmic heme-binding protein from Vibrio cholerae. Although we have previously identified HutZ as a heme-degrading enzyme [Uchida, T., et al. (2012) Chem. Commun. 48, 6741-6743], the heme transport protein for HutZ remained unknown. To identify the heme transport protein for HutZ, we focused on the heme utilization operon, hutWXZ. To this end, we constructed an expression system for HutX in Escherichia coli and purified it to homogeneity. An absorption spectral analysis demonstrated that HutX binds heme with a 1:1 stoichiometry and a dissociation constant of 7.4 nM. The crystal structure of HutX displays a fold similar to that of the homologous protein, ChuX, from E. coli O157:H7. A structural comparison of HutX and ChuX, and resonance Raman spectra of heme-HutX, suggest that the axial ligand of the ferric heme is Tyr90. The heme bound to HutX is transferred to HutZ with biphasic dissociation kinetics of 8.3 × 10(-2) and 1.5 × 10(-2) s(-1), values distinctly larger than those for transfer from HutX to apomyoglobin. Surface plasmon resonance experiments confirmed that HutX interacts with HutZ with a dissociation constant of ∼400 μM. These results suggest that heme is transferred from HutX to HutZ via a specific pro...Continue Reading

References

Feb 15, 1987·Analytical Biochemistry·E A Berry, B L Trumpower
Nov 22, 1994·Biochemistry·J SunP R Ortiz de Montellano
Jan 1, 1997·Free Radical Biology & Medicine·J Everse, N Hsia
Feb 1, 1997·Molecular Microbiology·A G Torres, S M Payne
Jun 9, 1999·Nature Structural Biology·P ArnouxM Czjzek
Apr 4, 2000·Current Opinion in Microbiology·C Wandersman, I Stojiljkovic
Jul 5, 2001·International Journal of Medical Microbiology : IJMM·V Braun
Oct 2, 2002·FEBS Letters·Volkmar Braun, Michael Braun
Feb 4, 2003·Nature Reviews. Molecular Cell Biology·José D Faraldo-Gómez, Mark S P Sansom
Oct 1, 1959·Biochimica Et Biophysica Acta·F W TEALE
Jun 19, 2004·Journal of Bacteriology·Elizabeth E WyckoffShelley M Payne
Nov 2, 2004·The Journal of Biological Chemistry·Yuichiro HigashimotoMasato Noguchi
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Dec 16, 2004·Journal of Inorganic Biochemistry·Thomas G Spiro, Ingar H Wasbotten
Mar 31, 2005·The Journal of Biological Chemistry·Takeshi UchidaTeizo Kitagawa
Dec 24, 2005·The Journal of Biological Chemistry·Julie M StevensStuart J Ferguson
Jan 12, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Elizabeth E WyckoffShelley M Payne
May 31, 2007·Natural Product Reports·Angela Wilks, Kimberly A Burkhard
Oct 6, 2007·Biochimica Et Biophysica Acta·Karla D Krewulak, Hans J Vogel
Jan 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Stefanie KriegWolfram Welte
Mar 26, 2009·Protein Science : a Publication of the Protein Society·Michael D L SuitsZongchao Jia
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Dec 1, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Ruiguang Ge, Xuesong Sun
May 26, 2012·Chemical Communications : Chem Comm·Takeshi UchidaKoichiro Ishimori
Jun 16, 2012·The Journal of Biological Chemistry·Célia Caillet-SaguyAnne Lecroisey
Nov 13, 2012·Nature Structural & Molecular Biology·Jae-Sung WooKaspar P Locher
May 2, 2014·Current Opinion in Chemical Biology·Heidi ContrerasCelia W Goulding

❮ Previous
Next ❯

Citations

May 31, 2016·International Journal of Molecular Sciences·Norifumi MurakiShigetoshi Aono
Jan 14, 2017·Biochemical and Biophysical Research Communications·Yuta WatanabeTakeshi Uchida
Apr 25, 2017·Biochemistry·Takeshi UchidaKoichiro Ishimori
Mar 6, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Takeshi UchidaKoichiro Ishimori
Apr 5, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Takeshi UchidaKoichiro Ishimori
Aug 10, 2017·Frontiers in Cellular and Infection Microbiology·Yingjie Li, Qingjun Ma
Nov 7, 2019·Archives of Biochemistry and Biophysics·Takeshi UchidaKoichiro Ishimori
May 14, 2021·Veterinary Microbiology·Ruijiao JiangYin Wang
Oct 28, 2019·Biochemistry·Liju G MathewWilliam N Lanzilotta
Oct 8, 2021·Journal of Cellular Physiology·Vasiliki-Dimitra C TsolakiAsterios S Tsiftsoglou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.