Mar 23, 2020

Integrated metabolomics and proteomics of symptomatic and early pre-symptomatic states of colitis

BioRxiv : the Preprint Server for Biology
E. ShimshoniPaola Turano

Abstract

Two murine models for colitis were used to study multi-level changes and derive molecular signatures of colitis onset and development. By combining metabolomics data on tissues and fecal extracts with proteomics data on tissues, we provide a comprehensive picture of the metabolic profile of acute and chronic states of the disease, and most importantly, of two early pre-symptomatic states. We show that, increased anaerobic glycolysis, accompanied by altered TCA cycle and oxidative phosphorylation, associates with inflammation-induced hypoxia taking place in colon tissues. We also demonstrate significant changes in the metabolomic profiles of fecal extracts in different colitis states, most likely associated with the dysbiosis characteristic of colitis, as well as the dysregulated tissue metabolism. Most remarkably, strong and distinctive tissue and fecal metabolomic signatures can be detected before onset of symptoms. These results highlight the diagnostic potential of global metabolomics for inflammatory diseases.

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Study
Calcium [EPC]
Zone
Ryanodine Receptor 3
Membrane
Calcium
Myocytes, Smooth Muscle
Calcium Signaling
Calcium ion
RYR3 gene

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Proceedings of the Royal Society of Medicine
T P Ormerod
Electrophoresis
Coral Barbas Arribas
BioTechniques
Ute Roessner, Jairus Bowne
FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Sophie ReadeChris S Probert
© 2020 Meta ULC. All rights reserved