Abstract
Elucidation of the biological roles of the mitochondrial and cytoplasmic hydroxyl radical (cyto OH) is hampered by the absence of site-specific OH scavengers. Earlier findings using cyto OH scavenger, TA293, indicated that cyto OH causes cellular senescence, and senescence-associated secretory phenotype (SASP) factors secreted from cells cause macrophage infiltration, inflammation, and apoptosis. However, we found that macrophage infiltration occurs before senescent cells appear. We therefore aimed to elucidate how cyto OH-induces macrophage activation and investigate the mechanism by which activated macrophages cause oxidative stress, inflammation, and apoptosis. In vivo imaging of pyocyanin- and TA293-treated, macrophage-depleted Toll-like receptor 4-knockout (TLR4-/-) OKD48- and IDOL-Tg mouse models were used to visualize oxidative stress and inflammation. SA-β-gal and TUNEL staining were used to detect cellular senescence and apoptosis. The mRNA expression of SASP factors were quantified by qRT-PCR. Activation mechanism of cyto OH-mediated macrophages was studied by an ex vivo analysis that created macrophage-activated oxidized phospholipids (OxPLs) using TLR4-/- mice. Cyto OH produced OxPLs that acted as TLR4 ligands, resu...Continue Reading
References
Jun 17, 2000·The American Journal of Medicine·J M McCord
May 23, 2001·Free Radical Biology & Medicine·S TurnbullD Allsop
Jan 25, 2006·Chemico-biological Interactions·M ValkoM Mazur
Dec 6, 2006·Free Radical Biology & Medicine·Michael Muller
Apr 22, 2008·Cell·Yumiko ImaiJosef M Penninger
Aug 25, 2009·Nature Medicine·Louise HeckerVictor J Thannickal
Jul 16, 2010·American Journal of Physiology. Renal Physiology·M SedeekR L Hébert
Jul 12, 2011·Growth Factors·Anna BiernackaNikolaos G Frangogiannis
Nov 15, 2011·Free Radical Biology & Medicine·Yanti OctaviaAn L Moens
Feb 23, 2012·Scientific Reports·Daisuke OikawaTakao Iwawaki
Jun 20, 2012·Current Medicinal Chemistry·V Sánchez-ValleN Méndez-Sánchez
Jan 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Shinichi EnamiAgustín J Colussi
Sep 5, 2014·Apoptosis : an International Journal on Programmed Cell Death·Li-Hua WuTakashi Kondo
Sep 30, 2014·Cellular & Molecular Immunology·Arvind PandaySanjay Batra
Jun 18, 2015·Science Signaling·Mateja Manček-KeberRoman Jerala
Nov 26, 2015·Scientific Reports·Takao IwawakiKen-ichi Yamamura
Mar 24, 2016·Oxidative Medicine and Cellular Longevity·Elena GammellaGaetano Cairo
Aug 1, 2014·Journal of Pulmonary & Respiratory Medicine·Leah VillegasEva Nozik-Grayck
Jun 28, 2016·Cell and Tissue Research·Kati Richter, Thomas Kietzmann
Dec 8, 2016·Biochemical and Biophysical Research Communications·Takahiro SakaiShinichi Hatta
Jan 21, 2017·Fundamental & Clinical Pharmacology·Yoshiji OhtaJunji Yoshino
Feb 12, 2017·Free Radical Biology & Medicine·Kazuhide FukuharaNoboru Hattori
May 20, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Wen-Wen DongLai Jiang
Jul 27, 2017·Scientific Reports·Sathnur PushpakumarUtpal Sen
Citations
Aug 6, 2020·MSystems·Ahmed A ElolimyLaxmi Yeruva
Apr 25, 2020·Cells·Pratap Karki, Konstantin G Birukov
Aug 3, 2021·Trends in Immunology·Jonathan J LiangClare E Bryant