Cytoplasmic Relocalization and Colocalization with Viroplasms of Host Cell Proteins, and Their Role in Rotavirus Infection

Journal of Virology
Poonam DhillonC Durga Rao

Abstract

Rotavirus replicates in the cytoplasm of infected cells in unique virus-induced cytoplasmic inclusion bodies called viroplasms (VMs), which are nucleated by two essential viral nonstructural proteins, NSP2 and NSP5. However, the precise composition of the VM, the intracellular localization of host proteins during virus infection, and their association with VMs or role in rotavirus growth remained largely unexplored. Mass spectrometry analyses revealed the presence of several host heterogeneous nuclear ribonucleoproteins (hnRNPs), AU-rich element-binding proteins (ARE-BPs), and cytoplasmic proteins from uninfected MA104 cell extracts in the pulldown (PD) complexes of the purified viroplasmic proteins NSP2 and NSP5. Immunoblot analyses of PD complexes from RNase-treated and untreated cell extracts, analyses of coimmunoprecipitation complexes using RNase-treated infected cell lysates, and direct binding assays using purified recombinant proteins further demonstrated that the interactions of the majority of the hnRNPs and ARE-BPs with viroplasmic proteins are RNA independent. Time course immunoblot analysis of the nuclear and cytoplasmic fractions from rotavirus-infected and mock-infected cells and immunofluorescence confocal micro...Continue Reading

References

Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D CaputA Cerami
Dec 1, 1989·Microbiological Reviews·M K Estes, J Cohen
Jan 20, 1988·Journal of Molecular Biology·B V PrasadW Chiu
Jan 1, 1980·The Journal of General Virology·B C AltenburgM K Estes
May 1, 1995·Molecular and Cellular Biology·Q Liu, G Dreyfuss
Nov 1, 1995·Trends in Biochemical Sciences·C Y Chen, A B Shyu
Nov 1, 1996·Ultrastructural Pathology·A TinariG Donelli
May 28, 1998·The Journal of Biological Chemistry·E AfoninaG N Pavlakis
Nov 20, 1998·The Journal of General Virology·I AfrikanovaO R Burrone
Mar 12, 1999·The Journal of General Virology·E FabbrettiO R Burrone
Jul 8, 1999·Current Opinion in Cell Biology·A M Krecic, M S Swanson
Apr 25, 2000·Journal of Molecular Biology·J H KimS K Jang
May 30, 2002·Virology·Victoria PandoSusana López
May 1, 1993·Trends in Cell Biology·S Piñol-Roma, G Dreyfuss
Mar 3, 2004·The Journal of General Virology·Catherine EichwaldOscar R Burrone
Apr 15, 2004·Molecular and Cellular Biology·Maria T Vassileva, Michael J Matunis
Aug 28, 2004·Journal of Virology·John W A RossenAlexandra W C Einerhand
Apr 16, 2005·The Journal of General Virology·Michela CampagnaOscar R Burrone
May 26, 2005·The Journal of General Virology·Tomás LópezCarlos F Arias
Jun 28, 2005·Nucleic Acids Research·Bing ZhangJay Snoddy
Jan 5, 2006·Nucleic Acids Research·Carine BarreauH Beverley Osborne
Oct 31, 2006·Memórias do Instituto Oswaldo Cruz·Claudio Cabral-Romero, Luis Padilla-Noriega
Apr 5, 2007·Nature Protocols·Andrej ShevchenkoMatthias Mann
Nov 14, 2008·Journal of Virology·Elizabeth L Pettit KnellerDouglas S Lyles

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Citations

Dec 14, 2018·Journal of Virology·Mariela DuarteDidier Poncet
Mar 25, 2019·Viruses·Raquel TenorioTerence S Dermody
Jul 26, 2019·ELife·Yasel Garcés SuárezCarlos F Arias
Nov 5, 2019·Frontiers in Cellular and Infection Microbiology·Aracelly Gaete-ArgelFernando Valiente-Echeverría
Apr 30, 2019·Wiley Interdisciplinary Reviews. RNA·Simon BoudreaultMartin Bisaillon
Aug 25, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Quentin NeversYves Gaudin
Dec 24, 2018·Trends in Microbiology·Usama AshrafNadia Naffakh
Oct 15, 2021·PLoS Pathogens·Nora LopezGonzalo de Prat-Gay

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