PMID: 14412546Jun 1, 1960Paper

Cytoplasmic ribonucleoprotein components of the Novikoff hepatoma

The Journal of Biophysical and Biochemical Cytology
E L KUFF, R F ZEIGEL

Abstract

Prominent nucleoprotein sedimentation boundaries were demonstrable in cytoplasmic extracts of Novikoff hepatoma. Fractionation of the homogenates by differential centrifugation or a density gradient method revealed that 65 to 75 per cent of the cytoplasmic ribonucleic acid was present in the form of free ribonucleoprotein particles. After purification by differential centrifugation in dilute buffer, the particles contained 37 per cent RNA, very little lipid, and no demonstrable membrane material. Ultracentrifugal boundaries corresponding to those seen in the original extracts were present, the main component having an s20, (w) of 81 S. Upon exposure to chelating agents, the particles dissociated through an intermediate component with sedimentation rate of 56 S to a final stage in which 46 and 28 S subunits were present in a weight ratio of 2:1. ATP and pyrophosphate were equally effective in causing dissociation. ADP was considerably less effective. Treatment of the purified particles with deoxycholate removed one-third of the protein and significantly altered the ultracentrifugal pattern. The particles now dissociated directly to the 46 and 28 S subunit when exposed to chelating agents. Upon electron microscopy, the 81 S parti...Continue Reading

References

Mar 1, 1956·Archives of Biochemistry and Biophysics·F C CHAO, H K SCHACHMAN
Mar 30, 1956·Annals of the New York Academy of Sciences·A J DALTON, M D FELIX
Mar 25, 1956·The Journal of Biophysical and Biochemical Cytology·G E PALADE, P SIEKEVITZ
Jul 25, 1956·The Journal of Biophysical and Biochemical Cytology·J BONNERJ VINOGRAD
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Sep 20, 1958·Nature·A TISSIERES, J D WATSON
Dec 1, 1958·Biochimica Et Biophysica Acta·P O TS'OJ VINOGRAD

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Citations

Aug 15, 1971·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M Aikawa, R T Cook
Jan 1, 1962·The Journal of Cell Biology·J CHAUVEAUJ SCHNEEBELI
Feb 15, 1974·European Journal of Biochemistry·M MeyerN Nanninga
Aug 25, 1971·European Journal of Biochemistry·B Mechler, B Mach
Dec 1, 1970·Archives of Biochemistry and Biophysics·F C Chao, H P Klein
Aug 1, 1970·Experimental Cell Research·M Phillips, R T Hersh
Jul 1, 1965·Canadian Journal of Biochemistry·J J GhoshK C Bhattacharyya
Jan 1, 1969·Progress in Biophysics and Molecular Biology·A S Spirin
Dec 1, 1963·Biochemical Pharmacology·R K DATTA, J J GHOSH
Feb 21, 1966·Biochimica Et Biophysica Acta·M L Petermann, A Pavlovec
Jan 4, 1966·Biochimica Et Biophysica Acta·E R Schwartz, M L Petermann
Jul 5, 1974·Journal of Molecular Biology·Y Ichikawa, H S Mason
Aug 1, 1966·Journal of Molecular Biology·C E ZomzelyC Provost
Feb 1, 1965·Journal of Molecular Biology·Y TASHIRO, D A YPHANTIS
Feb 1, 1965·Journal of Molecular Biology·Y TASHIRO, P SIEKEVITZ

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