Cytoplasmic wax ester accumulation during biofilm-driven substrate assimilation at the alkane--water interface by Marinobacter hydrocarbonoclasticus SP17

Research in Microbiology
Benjamin KleinRégis Grimaud

Abstract

During growth on n-alkanes, the marine bacterium Marinobacter hydrocarbonoclasticus SP17 formed a biofilm at the alkane-water interface. We showed that hexadecane degradation was correlated with biofilm development and that alkane uptake is localized in the biofilm but not in the bulk medium. Biofilms were observed in cultures on metabolizable n-alkanes (C8-C28) and n-alcohols (C12 and C16), but were formed neither on non-metabolizable alkanes (pristane, heptamethylnonane and n-C32) nor on inert substrata (glass, polystyrene and Permanox). This substratum specificity indicates that biofilm formation is determined by the presence of an interface between an insoluble substrate and the aqueous phase. Simultaneously with biofilm growth, planktonic cells were released from the biofilm. Detached cells were in a non-growing state, implying that the growing population was exclusively located within the biofilm. Planktonic and sessile cells exhibited differences in their ultrastructure and lipid content. Biofilm cells contained a large amount of wax esters (0.47mg/mg protein) in rounded or irregularly shaped cytoplasmic inclusions, whereas detached cells displayed rod-shaped inclusions and contained 5 times fewer wax esters (0.10mg/mg p...Continue Reading

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Citations

Jun 1, 2011·World Journal of Microbiology & Biotechnology·Sushma Deepthi ArliK C Patel
Sep 13, 2012·Chemosphere·Roger C PrinceTim J Nedwed
Nov 22, 2016·Environmental Microbiology·Manuel Espinosa-Urgel, Silvia Marqués
May 24, 2013·Microbiology and Molecular Biology Reviews : MMBR·Annika Röttig, Alexander Steinbüchel
Jul 26, 2019·Environmental Microbiology Reports·Pierre SivadonRégis Grimaud
May 16, 2019·Microbial Biotechnology·Patricia MarínSilvia Marqués
Dec 7, 2018·New Biotechnology·Mark PannekensRainer U Meckenstock
Dec 19, 2020·Biotechnology Advances·Laura K MartinIan P Thompson

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