Cytoprotection, proliferation and epidermal differentiation of adipose tissue-derived stem cells on emu oil based electrospun nanofibrous mat

Experimental Cell Research
Younes Pilehvar-SoltanahmadiNosratollah Zarghami

Abstract

Electrospun nanofibrous scaffolds containing natural substances with wound healing properties such as Emu oil (EO) may have a great potential for increasing the efficiency of stem cell-based skin bioengineering. For this purpose, EO blended PCL/PEG electrospun nanofibrous mats were successfully fabricated and characterized using FE-SEM, FTIR and Universal Testing Machine. The efficiency of the scaffolds in supporting the adherence, cytoprotection, proliferation and differentiation of adipose tissue-derived stem cells (ADSCs) to keratinocyte was evaluated. GC/MS and HPLC were used to determine the composition of pure EO, which revealed to be mainly fatty acids and carotenoids. FE-SEM and cell proliferation assays showed that adhesion and proliferation of ADSCs on EO-PCL/PEG nanofibers was significantly higher than on PCL/PEG nanofibers. Additionally, EO-PCL/PEG nanofibers with free radical scavenging properties conferred a cytoprotective effect against cell-damaging free radicals, while the ability to support cell adhesion and growth was maintained or even improved. Immunostaining of ADSCs on EO-PCL/PEG nanofibers confirmed the change in morphology of ADSCs from spindle to polygonal shape suggesting their differentiation toward ...Continue Reading

Citations

Jul 12, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Mehdi DadashpourMohammad Nouri
Jan 23, 2018·Biopreservation and Biobanking·Sepideh Jalilzadeh-TabriziNosratollah Zarghami
Aug 24, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Reza ZamaniNosratollah Zarghami
Jun 5, 2018·Stem Cells International·Jeong-Kee YoonHak-Joon Sung
Nov 28, 2018·Journal of Cellular Physiology·Behnaz TaheriNosratollah Zarghami
Feb 6, 2020·Journal of Cellular Physiology·Nazila Fathi MaroufiReza Rahbarghazi
Mar 21, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Leila FaramarziNosratollah Zarghami
Jan 23, 2020·Hormone Molecular Biology and Clinical Investigation·Vahid VahedianNazila Fathi Maroufi
Jun 18, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Akram Firouzi-AmandiYounes Pilehvar-Soltanahmadi
Jul 20, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hassan MellatyarNosratollah Zarghami

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