Cytoprotective Activities of Milk Thistle Seed Oil Used in Traditional Tunisian Medicine on 7-Ketocholesterol and 24S-Hydroxycholesterol-Induced Toxicity on 158N Murine Oligodendrocytes

Antioxidants
Wiem MeddebGérard Lizard

Abstract

The Asteraceae family is economically very important, because many of these plants are grown mainly for their food value, such as lettuce (Lactuca), chicory (Cichorium), and sunflower (Heliantus aminus). One of the typical properties of this family, which includes milk thistle (Sylibum marianum), is the richness of the oil in various compounds (flavonoids, alkaloids, tocopherols, and unsaturated fatty acids). Currently, and for the coming decades, age-related diseases, including neurodegenerative diseases, are a major public health problem. Preventing their appearance or opposing their evolution is a major objective. In this context, the cytoprotective activities of milk thistle seed oil produced in Tunisia were studied on the 158N model using 7-ketocholesterol (7KC) and 24S-hydroxycholesterol (24S) as cytotoxic agents. 7KC and 24S were used because they can be increased in the brain and body fluids of patients with major age-related neurodegenerative diseases, such as Alzheimer's and Parkinson's diseases. In order to evaluate the cytoprotective properties of milk thistle seed oil, complementary techniques of microscopy, flow cytometry, and biochemistry were used. The chemical composition of milk thistle seed oil has also been ...Continue Reading

References

Jun 7, 1985·Science·M F BalandrinW H Bollinger
Jul 18, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·T Moilanen, T Nikkari
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·D LütjohannI Björkhem
Jan 5, 2000·Physiological Reviews·G J Schroepfer
Jan 5, 2000·The American Journal of Clinical Nutrition·W E Connor
Apr 2, 2002·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·A ZaouiM Hassar
Jun 13, 2002·Journal of Molecular Neuroscience : MN·Smita KitturDilip S Kittur
Aug 30, 2002·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·H TapieroK D Tew
Sep 7, 2002·International Journal of Cancer. Journal International Du Cancer·Hiroyuki KohnoHideki Mori
Mar 26, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jean-Marie ZahmGérard Lizard
Apr 3, 2003·Biochemical and Biophysical Research Communications·Serge MonierGérard Lizard
Apr 15, 2003·The Proceedings of the Nutrition Society·Gerald RimbachPeter D Weinberg
Aug 29, 2003·The Journal of Surgical Research·Shung-Haur YangJen-Hwey Chiu
Oct 29, 2003·Antioxidants & Redox Signaling·Denham Harman
Sep 21, 2004·Annual Review of Plant Biology·Pierre Benveniste
Jan 15, 2008·Cell·Beth Levine, Guido Kroemer
May 1, 2008·The Journal of Biological Chemistry·Paul A GrimsrudDavid A Bernlohr
Aug 23, 2008·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A VejuxG Lizard
Feb 10, 2009·Current Medicinal Chemistry·Barbara SotteroGiuseppe Poli
Apr 7, 2009·The Journal of Nutritional Biochemistry·Sinéad LordanNora M O'Brien
Sep 28, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A Otaegui-ArrazolaI Astiasarán
Apr 26, 2011·Chemistry and Physics of Lipids·Valerio Leoni, Claudio Caccia
Jun 15, 2011·Free Radical Biology & Medicine·Maret G Traber, Jan F Stevens
Jun 28, 2011·Chemistry and Physics of Lipids·Luigi Iuliano
Oct 26, 2011·Current Neurovascular Research·Elodie VandenhauteMarie-Pierre Dehouck
Sep 8, 2012·Research in Veterinary Science·Raffaella RossiCarlo Corino
Jun 19, 2013·The American Journal of Pathology·Chaoyong HeZhonglin Xie
Sep 12, 2013·Redox Biology·Giuseppe PoliGabriella Leonarduzzi
Sep 28, 2014·Experimental Eye Research·Ignacio R RodriguezChristine A Curcio
Oct 12, 2014·Ageing Research Reviews·Amira ZarroukGérard Lizard

❮ Previous
Next ❯

Citations

Jun 25, 2020·British Journal of Pharmacology·Thomas NuryGérard Lizard
Apr 9, 2020·International Journal of Molecular Sciences·Thomas NuryAnne Vejux

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
electrophoresis
flow cytometry

Software Mentioned

GraphPad Prism
EZChrom Elite
Flomax
GraphPad
Flowjo
Image Lab

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.

Parkinson's Disease & Autophagy

Autophagy leads to degradation of damaged proteins and organelles by the lysosome. Impaired autophagy has been implicated in several diseases. Here is the role of autophagy in Parkinson’s disease.

Parkinson's Disease & Autophagy (MDS)

Autophagy leads to degradation of damaged proteins and organelles by the lysosome. Impaired autophagy has been implicated in several diseases. Here is the role of autophagy in Parkinson’s disease.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.