Cytoprotective activity of minocycline includes improvement of mitochondrial coupling: the importance of minocycline concentration and the presence of VDAC.

Journal of Bioenergetics and Biomembranes
Andonis KarachitosHanna Kmita

Abstract

Available data indicate that minocycline, an antibiotic of the tetracycline family, has cytoprotective properties due to a direct interaction with mitochondria. Yet, the data in the case of isolated mitochondria suggest discrepant or even detrimental effect(s) of the interaction. We have studied the cytoprotective activity displayed by minocycline in the case of the yeast Saccharomyces cerevisiae cells pretreated with H₂O₂. We demonstrated that the activity of minocycline required the presence of VDAC (voltage-dependent anion-selective channel) and provided distinct improvement of mitochondrial coupling. In the case of isolated mitochondria, we verified that minocycline exhibited uncoupler activity when applied in micromolar concentrations. However, when added in nanomolar concentrations, minocycline was able to improve the level of coupling for isolated mitochondria. The coupling improvement effect was observed in mitochondria containing VDAC but not in Δpor1 mitochondria (depleted of VDAC1, termed here VDAC) and in both types of mitoplasts. Thus, properly low concentrations of minocycline within the cell in the vicinity of VDAC-containing mitochondria enable the improvement of energy coupling of mitochondria that contributes ...Continue Reading

References

Aug 31, 1990·Biochemical and Biophysical Research Communications·J MichejdaG J Lauquin
Jan 1, 1971·Archiv für Mikrobiologie·C SpencerR V Brunt
Jan 22, 1998·The Journal of Membrane Biology·A C LeeM Colombini
Sep 12, 1998·The Journal of Biological Chemistry·J SongM Colombini
Dec 5, 1998·Journal of Clinical Periodontology·B N VandekerckhoveD van Steenberghe
Nov 24, 2004·European Journal of Pharmacology·Sylvie CornetMichel Auguet
Nov 15, 2006·Current Opinion in Neurology·Kevin K W WangRonald L Hayes
Apr 6, 2007·Journal of Virology·Alan C JacksonJohn P Rossiter
May 1, 2007·Proteomics·Francesca MagheriniAlessandra Modesti
Jul 14, 2007·Current Drug Delivery·J JordanM F Galindo
Nov 4, 2008·Behavioural Brain Research·Hye-Sun Kim, Yoo-Hun Suh
Nov 11, 2008·CNS & Neurological Disorders Drug Targets·Omar M E Abdel-Salam
Nov 11, 2008·Recent Patents on CNS Drug Discovery·Joanna S FongDeRen Huang
Dec 2, 2008·Biochemical Pharmacology·Anne GieselerPeter Kreutzmann
Feb 27, 2009·The FEBS Journal·Kathleen KupschPeter Schönfeld
Aug 18, 2009·Biochemical Pharmacology·Eva Maria Garcia-MartinezJoaquín Jordán
Oct 8, 2009·BMC Neuroscience·Noriyuki MatsukawaCesar V Borlongan
Dec 18, 2009·CNS & Neurological Disorders Drug Targets·Li LuXiaomin Wang
Dec 25, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Roland MånssonEskil Elmér
Feb 25, 2010·Journal of Bioenergetics and Biomembranes·Yuri N AntonenkoBoris F Krasnikov
May 11, 2010·Cellular and Molecular Neurobiology·Peter KreutzmannKathleen Kupsch
Aug 11, 2010·Archives of Neurology·Jennifer M PlaneWenbin Deng
Aug 14, 2010·Stroke; a Journal of Cerebral Circulation·Susan C FaganDavid C Hess
Oct 13, 2010·BMC Neurology·Carlo ParibelloDouglas W Ethell
Mar 19, 2011·Annals of Neurology·Kenji Hashimoto
May 14, 2011·Journal of Neuroimmunology·Xiaohong ChenLili Ma
Sep 3, 2011·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Maria D Cuenca-LopezJoaquin Jordán

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