Cytoprotective effect of curcumin in human proximal tubule epithelial cells exposed to shiga toxin

Biochemical and Biophysical Research Communications
A SoodH Trachtman

Abstract

We conducted the following experiments to determine whether curcumin, an antioxidant compound extracted from the spice tumeric, inhibits cell death induced by Shiga toxin (Stx) 1 and 2 in HK-2 cells, a human proximal tubule cell line. Cells were incubated for 24-48 h with Stx1 or Stx2, 0-100 ng/ml. Test media contained either no further additives or 10-50 microM curcumin. Exposure to Stx1 and Stx2, 100 ng/ml, reduced cell viability to approximately 25% of control values after 24 h and 20 microM curcumin restored viability to nearly 75% of control. Cell staining confirmed that Stx1 and Stx2-induced damage in HK-2 cells involved a combination of apoptosis and necrosis. Thus, Stx1 caused apoptosis and necrosis in 12.2 +/- 2.2 and 12.7 +/- 0.9% of HK-2 cells, respectively. Similarly, Stx2 caused apoptosis and necrosis in 13.4 +/- 2.1 and 9.0 +/- 0.5% of HK-2 cells, respectively. Addition of 20 microM curcumin decreased the extent of apoptosis and necrosis to 2.9 +/- 2.0 and 3.8 +/- 0.2%, respectively in the presence of Stx1 and to 3.0 +/- 2.1 and 3.9 +/- 0.3%, respectively, for Stx2 (P < 0.01). Stx-induced apoptosis and its inhibition by curcumin were confirmed by DNA gel electrophoresis and by an assay for fragmentation. The prote...Continue Reading

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Citations

Mar 19, 2011·Biological & Pharmaceutical Bulletin·Fang ZhongWeiming Wang
Mar 10, 2010·Future Microbiology·Vernon L Tesh
Mar 28, 2006·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Howard TrachtmanPravin C Singhal
Mar 17, 2004·Free Radical Biology & Medicine·Heidi LigeretDidier Morin
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Sep 16, 2008·Molecular Nutrition & Food Research·Vittorio CalabreseAlbena T Dinkova-Kostova
Apr 15, 2015·Pesticide Biochemistry and Physiology·Anoop Kumar, Neelima Sharma
Dec 21, 2016·Chemistry Central Journal·Reem Ibrahim Al-WabliWafaa Abdou Zaghary

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis