PMID: 22358095Feb 24, 2012Paper

Cytoprotective effects of CSTMP, a novel stilbene derivative, against H2O2-induced oxidative stress in human endothelial cells

Pharmacological Reports : PR
Li ZhaiXiu-Li Guo

Abstract

A novel stilbene derivative, (E)-2-(2-chlorostyryl)-3,5,6-trimethylpyrazine (CSTMP), was designed and synthesized based on the pharmacophores of tetramethylpyrazine (TMP) and resveratrol (RES). In the present study, we investigated the protective effects of CSTMP on vascular endothelial cells under oxidative stress and elucidated its molecular mechanisms. The radical scavenging activity of CSTMP was assessed by the DPPH test. Human Umbilical Vein Endothelial Cells (HUVECs) were exposed to 150 μM hydrogen peroxide (H(2)O(2)) for 12 h, resulting in a decrease of cell viability assessed by the MTT assay and an increase of apoptotic cells assessed by the nuclear staining assay and flow cytometry. The activities of lactate dehydrogenase (LDH), superoxide dismutase (SOD) and nitric oxide synthase (NOS) and the contents of malondialdehyde (MDA), reduced glutathione (GSH) and nitric oxide (NO) in cells were determined by commercial kits. The expression levels of pro-apoptotic factor caspase-3 and anti-apoptotic signal ERK1/2 were detected by western blot. The results showed that CSTMP had a moderate anti-oxidative effect against the DPPH test, which was less than RES. Co-incubation with CSTMP increased the cell viability, markedly redu...Continue Reading

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Citations

Apr 10, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Konstantinos M KasiotisSerkos A Haroutounian
Sep 18, 2012·Apoptosis : an International Journal on Programmed Cell Death·Himani AnandSant Prakash
Apr 11, 2014·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·Wei-Guo LiuXiu-Li Guo
Jul 30, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jiexia ZhangWeiqiang Yin
Oct 25, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xing SunYan Ding
Aug 2, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Junping GuoJianping Pan
Jul 31, 2013·The American Journal of Chinese Medicine·Štefan TóthJuraj Guzy
Jul 10, 2018·International Journal of Immunopathology and Pharmacology·Chun-Mei WangBo Bai
May 3, 2014·Molecular Medicine Reports·Mingshun ZhangChunbing Zhang
Nov 14, 2019·CNS & Neurological Disorders Drug Targets·Khadga RajShamsher Singh
Oct 15, 2020·International Journal of Molecular Sciences·Chen Zeng, Shiling Feng
Apr 12, 2014·Molecular Pharmaceutics·Chun-Hui LiangSi-Yuan Zhou

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